Anti-GSS Antibody (CAB13495)

Antibody Name:	Anti-GSS Antibody
Antibody SKU:	CAB13495
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-474 of human GSS (NP_000169.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SW480, K-562, BT-474, Raji, Mouse kidney, Mouse liver, Rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-474 of human GSS (NP_000169.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MATN WGSL LQDK QQLE ELAR QAVD RALA EGVL LRTS QEPT SSEV VSYA PFTL FPSL VPSA LLEQ AYAV QMDF NLLV DAVS QNAA FLEQ TLSS TIKQ DDFT ARLF DIHK QVLK EGIA QTVF LGLN RSDY MFQR SADG SPAL KQIE INTI SASF GGLA SRTP AVHR HVLS VLSK TKEA GKIL SNNP SKGL ALGI AKAW ELYG SPNA LVLL IAQE KERN IFDQ RAIE NELL ARNI HVIR RTFE DISE KGSL DQDR RLFV DGQE IAVV YFRD GYMP RQYS LQNW EARL LLER SHAA KCPD IATQ LAGT KKVQ QELS RPGM LEML LPGQ PEAV ARLR ATFA GLYS LDVG EEGD QAIA EALA APSR FVLK PQRE GGGN NLYG EEMV QALK QLKD SEER ASYI LMEK IEPE PFEN CLLR PGSP ARVV QCIS ELGI FGVY VRQE KTLV MNKH VGHL LRTK AIEH ADGG VAAG VAVL DNPY PV
Gene ID:	2937
Uniprot:	P48637
Cellular Location:
Calculated MW:	40kDa/52kDa
Observed MW:	52kDa

Synonyms:

GSS, GSHS, HEL-S-64p, HEL-S-88n

Background:

Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency.

UniProt Protein Function:	GSS: Defects in GSS are the cause of glutathione synthetase deficiency (GSS deficiency); also known as 5- oxoprolinuria or pyroglutamic aciduria. It is a severe form characterized by an increased rate of hemolysis and defective function of the central nervous system. Defects in GSS are the cause of glutathione synthetase deficiency of erythrocytes (GLUSYNDE)[MIM:231900]. Glutathione synthetase deficiency of erythrocytes is a mild form causing hemolytic anemia. Belongs to the eukaryotic GSH synthase family.
UniProt Protein Details:	Protein type:EC 6.3.2.3; Other Amino Acids Metabolism - glutathione; Ligase Chromosomal Location of Human Ortholog: 20q11.2 Cellular Component: cytosol Molecular Function:protein homodimerization activity; glutathione binding; glycine binding; magnesium ion binding; glutathione synthase activity; ATP binding Biological Process: amino acid metabolic process; response to nutrient levels; nervous system development; response to cadmium ion; xenobiotic metabolic process; glutathione biosynthetic process; response to oxidative stress; response to amino acid stimulus; aging Disease: Glutathione Synthetase Deficiency; Glutathione Synthetase Deficiency Of Erythrocytes, Hemolytic Anemia Due To
NCBI Summary:	Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency. [provided by RefSeq, Jul 2008]
UniProt Code:	P48637
NCBI GenInfo Identifier:	1346191
NCBI Gene ID:	2937
NCBI Accession:	P48637.1
UniProt Secondary Accession:	P48637,Q4TTD9, B2R697, B6F210, E1P5P9,
UniProt Related Accession:	P48637
Molecular Weight:	474
NCBI Full Name:	Glutathione synthetase
NCBI Synonym Full Names:	glutathione synthetase
NCBI Official Symbol:	GSS
NCBI Official Synonym Symbols:	GSHS; HEL-S-64p; HEL-S-88n
NCBI Protein Information:	glutathione synthetase; GSH-S; GSH synthetase; glutathione synthase; epididymis secretory sperm binding protein Li 64p; epididymis secretory sperm binding protein Li 88n
UniProt Protein Name:	Glutathione synthetase
UniProt Synonym Protein Names:	Glutathione synthase
Protein Family:	Glutathione synthetase
UniProt Gene Name:	GSS
UniProt Entry Name:	GSHB_HUMAN

	Western blot analysis of extracts of various cell lines, using GSS antibody (CAB13495) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded mouse lung using GSS antibody (CAB13495) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of A549 cells using GSS antibody (CAB13495). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-GSS Antibody (CAB1069)	Human GSS (Glutathione synthetase) ELISA Kit (HUFI03449)
Anti-GSS Antibody (CAB14535)[KO Validated]
Anti-Glutathione Synthetase Antibody (CAB11557)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)