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[KO Validated] Glutathione Synthetase (GSS) Rabbit Polyclonal Antibody (CAB14535)

SKU:
CAB14535
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism
  • KO Validated Antibodies 1 Anti-GSS Antibody CAB14535KO Validated
  • KO Validated Antibodies 1 Anti-GSS Antibody CAB14535KO Validated
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:[KO Validated] Glutathione Synthetase (GSS) Rabbit Polyclonal Antibody
SKU:CAB14535
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 373-474 of human Glutathione Synthetase (Glutathione Synthetase (GSS)) (NP_000169.1).
Sequence:NLYG EEMV QALK QLKD SEER ASYI LMEK IEPE PFEN CLLR PGSP ARVV QCIS ELGI FGVY VRQE KTLV MNKH VGHL LRTK AIEH ADGG VAAG VAVL DNPY PV
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200
Synonyms:GSHS; HEL-S-64p; HEL-S-88n; S)
Positive Sample:293T,SH-SY5Y,Mouse liver,Mouse kidney,Rat lung
Conjugate:Unconjugated
Cellular Localization:cytosol, extracellular exosome
Calculated MW:52kDa
Observed MW:52kDa

Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency.

Purification Method:Affinity purification
Gene ID:2937
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from wild type (WT) and Glutathione Synthetase (Glutathione Synthetase (GSS)) knockout (KO) 293T cells, using [KO Validated] Glutathione Synthetase (GSS) Rabbit pAb (CAB14535) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.

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