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Anti-GTF2F2 Antibody (CAB13497)

SKU:
CAB13497
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
  • Epigenetics and Nuclear Signaling Antibodies 1 Anti-GTF2F2 Antibody CAB13497
  • Epigenetics and Nuclear Signaling Antibodies 1 Anti-GTF2F2 Antibody CAB13497
  • Epigenetics and Nuclear Signaling Antibodies 1 Anti-GTF2F2 Antibody CAB13497
  • Epigenetics and Nuclear Signaling Antibodies 1 Anti-GTF2F2 Antibody CAB13497
€139 - €419
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Description

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Antibody Name:Anti-GTF2F2 Antibody
Antibody SKU:CAB13497
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-249 of human GTF2F2 (NP_004119.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:HepG2, Mouse thymus, Mouse skeletal muscle
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-249 of human GTF2F2 (NP_004119.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAER GELD LTGA KQNT GVWL VKVP KYLS QQWA KASG RGEV GKLR IAKT QGRT EVSF TLNE DLAN IHDI GGKP ASVS APRE HPFV LQSV GGQT LTVF TESS SDKL SLEG IVVQ RAEC RPAA SENY MRLK RLQI EESS KPVR LSQQ LDKV VTTN YKPV ANHQ YNIE YERK KKED GKRA RADK QHVL DMLF SAFE KHQY YNLK DLVD ITKQ PVVY LKEI LKEI GVQN VKGI HKNT WELK PEYR HYQG EEKS D
Gene ID:2963
Uniprot:P13984
Cellular Location:Nucleus
Calculated MW:28kDa
Observed MW:28kDa
Synonyms:GTF2F2, BTF4, RAP30, TF2F2, TFIIF
Background:
UniProt Protein Function:GTF2F2: TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation. This subunit shows ATP-dependent DNA- helicase activity. Belongs to the TFIIF beta subunit family.
UniProt Protein Details:

Protein type:Helicase; EC 3.6.4.12; Nuclear receptor co-regulator; Transcription initiation complex

Chromosomal Location of Human Ortholog: 13q14

Cellular Component: nucleoplasm; microtubule cytoskeleton; transcription factor TFIIF complex; nucleus

Molecular Function:protein binding; DNA binding; ATP-dependent helicase activity; ATP binding

Biological Process: nuclear mRNA splicing, via spliceosome; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; mRNA capping; viral reproduction; regulation of transcription, DNA-dependent; positive regulation of viral transcription; RNA splicing; RNA elongation from RNA polymerase II promoter; gene expression

UniProt Code:P13984
NCBI GenInfo Identifier:464519
NCBI Gene ID:2963
NCBI Accession:P13984.2
UniProt Secondary Accession:P13984,Q5W0H3, A6NNS5,
UniProt Related Accession:P13984
Molecular Weight:249
NCBI Full Name:General transcription factor IIF subunit 2
NCBI Synonym Full Names:general transcription factor IIF, polypeptide 2, 30kDa
NCBI Official Symbol:GTF2F2  
NCBI Official Synonym Symbols:BTF4; RAP30; TF2F2; TFIIF  
NCBI Protein Information:general transcription factor IIF subunit 2; TFIIF-beta; ATP-dependent helicase GTF2F2; transcription initiation factor RAP30; general transcription factor IIF 30 kDa subunit; transcription initiation factor IIF subunit beta
UniProt Protein Name:General transcription factor IIF subunit 2
UniProt Synonym Protein Names:ATP-dependent helicase GTF2F2; General transcription factor IIF 30 kDa subunit; Transcription initiation factor IIF subunit beta; TFIIF-beta; Transcription initiation factor RAP30
Protein Family:General transcription factor
UniProt Gene Name:GTF2F2  
UniProt Entry Name:T2FB_HUMAN
Anti-GTF2F2 Antibody (CAB13497)Western blot analysis of extracts of various cell lines, using GTF2F2 antibody (CAB13497) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
Anti-GTF2F2 Antibody (CAB13497)Immunohistochemistry of paraffin-embedded human colon using GTF2F2 antibody (CAB13497) at dilution of 1:200 (40x lens).
Anti-GTF2F2 Antibody (CAB13497)Immunohistochemistry of paraffin-embedded human kidney cancer using GTF2F2 antibody (CAB13497) at dilution of 1:200 (40x lens).
Anti-GTF2F2 Antibody (CAB13497)Immunofluorescence analysis of A549 cells using GTF2F2 antibody (CAB13497).

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