The GTF2F2 Antibody (CAB5826) is a high-quality antibody developed for reliable detection and analysis of target proteins. Predicted to enable RNA polymerase II general transcription initiation factor activity. Involved in transcription by RNA polymerase II. Located in microtubule cytoskeleton and nucleoplasm. Part of transcription preinitiation complex. RRID AB_2766578 Gene ID 2963 Swiss Prot Synonym BTF4; RAP30; TF2F2; TFIIF; GTF2F2
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
GTF2F2 Antibody
SKU:
CAB5826
Size:
100μL, 20μL
Reactivity:
Human
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:2000
IF
/
ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BTF4, RAP30, TF2F2, TFIIF, GTF2F2
Positive Sample:
SW480, 293T, HepG2, HL-60, A-549
Cellular Localization:
Nucleus.
Calculated MW:
28kDa
Observed MW:
29kDa
Predicted to enable RNA polymerase II general transcription initiation factor activity. Involved in transcription by RNA polymerase II. Located in microtubule cytoskeleton and nucleoplasm. Part of transcription preinitiation complex. RRID AB_2766578 Gene ID 2963 Swiss Prot Synonym BTF4; RAP30; TF2F2; TFIIF; GTF2F2
Purification Method:
Affinity purification
Gene ID:
2963
RRID:
AB_2766578
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using GTF2F2 Rabbit pAb (CAB5826) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of A549 cells using GTF2F2 Rabbit pAb (CAB5826). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
