Anti-HK2 Antibody (CAB0994)

Antibody Name:	Anti-HK2 Antibody [KO Validated]
Antibody SKU:	CAB0994
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human HK2 (NP_000180.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SH-SY5Y, HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human HK2 (NP_000180.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MIAS HLLA YFFT ELNH DQVQ KVDQ YLYH MRLS DETL LEIS KRFR KEME KGLG ATTH PTAA VKML PTFV RSTP DGTE HGEF LALD LGGT NFRV LWVK VTDN GLQK VEME NQIY AIPE DIMR
Gene ID:	3099
Uniprot:	P52789
Cellular Location:	Mitochondrion outer membrane
Calculated MW:	102kDa
Observed MW:	102KDa

Synonyms:

HK2, HKII, HXK2, hexokinase-2

Background:

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.

UniProt Protein Function:	HK2: a glycolytic enzyme that catalyzes the reaction ATP + D-hexose = ADP + D-hexose 6-phosphate. The first and rate-limiting step in glycosis, a pathway that produces energy in the form of ATP from glucose. An allosteric enzyme inhibited by its product glucose-6-phosphate (Glc-6-P). The predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle. Acts as a ""glucose sensor"" by trapping glucose inside the cell by catalyzing its phosphorylation to produce Glc-6-P. In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase). Genetic variations of HK2 do not appear to contribute to NIDDM.
UniProt Protein Details:	Protein type:Kinase, other; EC 2.7.1.1; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Mitochondrial; Carbohydrate Metabolism - starch and sucrose; Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - galactose Chromosomal Location of Human Ortholog: 2p13 Cellular Component: cytosol; membrane; mitochondrial outer membrane Molecular Function:fructokinase activity; glucokinase activity; hexokinase activity; mannokinase activity; protein binding Biological Process: apoptotic mitochondrial changes; cell glucose homeostasis; glucose transport; glycolysis
NCBI Summary:	Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq, Apr 2009]
UniProt Code:	P52789
NCBI GenInfo Identifier:	56405344
NCBI Gene ID:	3099
NCBI Accession:	P52789.2
UniProt Secondary Accession:	P52789,Q8WU87, Q9UN82, D6W5J2,
UniProt Related Accession:	P52789
Molecular Weight:	102,380 Da
NCBI Full Name:	Hexokinase-2
NCBI Synonym Full Names:	hexokinase 2
NCBI Official Symbol:	HK2
NCBI Official Synonym Symbols:	HKII; HXK2
NCBI Protein Information:	hexokinase-2
UniProt Protein Name:	Hexokinase-2
UniProt Synonym Protein Names:	Hexokinase type II; HK II; Muscle form hexokinase
Protein Family:	Probable histidine kinase
UniProt Gene Name:	HK2
UniProt Entry Name:	HXK2_HUMAN

	Western blot analysis of extracts of various cell lines, using HK2 antibody (CAB0994) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Western blot analysis of extracts from normal (control) and HK2 knockout (KO) HeLa cells, using HK2 antibody (CAB0994) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human breast cancer using HK2 antibody (CAB0994) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon using HK2 antibody (CAB0994) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse heart using HK2 antibody (CAB0994) at dilution of 1:200 (40x lens).

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)