The Human Activin A Receptor Type I (ACVR1) ELISA Kit is a cutting-edge tool designed for the precise quantification of ACVR1 levels in human serum, plasma, and cell culture supernatants. This innovative kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results, making it an invaluable asset for a wide range of research applications.ACVR1 is a key receptor involved in the activin signaling pathway, playing a vital role in various biological processes such as cell growth, differentiation, and development.
Dysregulation of ACVR1 has been linked to a variety of diseases including cancer, bone disorders, and inflammatory conditions, highlighting its significance as a potential therapeutic target and diagnostic marker.With its advanced technology and reliability, the Human ACVR1 ELISA Kit offers researchers a powerful tool for studying the role of ACVR1 in disease pathology and developing novel treatment strategies. Elevate your research with the precision and accuracy of the Human ACVR1 ELISA Kit from Assay Genie.
Product Name:
Human Activin A Receptor Type I (ACVR1) ELISA Kit
Product Code:
HUDL00052
Size:
96 Assays
Target:
ACVR1
Synonyms:
ACVR1A, ALK2, ACVRLK2, TSRI, FOP, SKR1, ACTRI, Activin receptor-like kinase 2, Serine/threonine-protein kinase receptor R1, TGF-B superfamily receptor type I
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
