Human Angiogenesis Array (20 targets)

Product Sku:	SARB0019
Size:	2, 4, or 8
Species Detected:	Human
Number of Targets Detected:	20
Gene Symbols:	ANG, CCL2, CCL5, CXCL1, CXCL2, CXCL3, CXCL5, CXCL8, EGF, FGF2\|FIGF, IFNG, IGF1, IL6, LEP, PDGFB, PGF, TGFB1, THPO, TIMP1, TIMP2, TPO, VEGFA
Compatible Sample Types:	Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:	Sandwich-based
Method of Detection:	Chemiluminescence
Pathway:
Quantitative/Semi-Quantitative:	Semi-Quantitative
Solid Support:	Membrane
Suggested Application(s):	Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process; Detecting Nephrotoxicity
Storage/Stability:	For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Hover over target for more information
Angiogenin Gene Symbol: ANG Gene ID: 283 Accession #: P03950	EGF Gene Symbol: EGF Gene ID: 1950 Accession #: P01133	ENA-78 (CXCL5) Gene Symbol: CXCL5 Gene ID: 6374 Accession #: P42830	bFGF Gene Symbol: FGF2 Gene ID: 2247 Accession #: P09038	GRO alpha/beta/gamma Gene Symbol: CXCL1/CXCL2/CXCL3 Gene ID: 2919/2920/2921 Accession #: P09341/P19875/P19876
IFN-gamma Gene Symbol: IFNG Gene ID: 3458 Accession #: P01579	IGF-1 Gene Symbol: IGF1 Gene ID: 3479 Accession #: P05019	IL-6 Gene Symbol: IL6 Gene ID: 3569 Accession #: P05231	IL-8 (CXCL8) Gene Symbol: IL8 Gene ID: 3576 Accession #: P10145	Leptin Gene Symbol: LEP Gene ID: 3952 Accession #: P41159
MCP-1 (CCL2) Gene Symbol: CCL2 Gene ID: 6347 Accession #: P13500	PDGF-BB Gene Symbol: PDGFB Gene ID: 5155 Accession #: P01127	PLGF Gene Symbol: PGF Gene ID: 5228 Accession #: P49763	RANTES (CCL5) Gene Symbol: CCL5 Gene ID: 6352 Accession #: P13501	TGF beta 1 Gene Symbol: TGFB1 Gene ID: 7040 Accession #: P01137
TIMP-1 Gene Symbol: TIMP1 Gene ID: 7076 Accession #: P01033	TIMP-2 Gene Symbol: TIMP2 Gene ID: 7077 Accession #: P16035	Thrombopoietin (TPO) Gene Symbol: THPO Gene ID: 7066 Accession #: P40225	VEGF-A Gene Symbol: VEGFA Gene ID: 7422 Accession #: P15692	VEGF-D Gene Symbol: FIGF Gene ID: 2277 Accession #: O43915

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

1.	Block membranes
2.	Incubate with Sample
3.	Incubate with Biotinylated Detection Antibody Cocktail
4.	Incubate with HRP-Conjugated Streptavidin
5.	Incubate with Detection Buffers
6.	Image with chemiluminescent imaging system
7.	Perform densitometry and analysis