Description
system_update_altDatasheet
Human Apoptosis Array (43 targets) (SARB0021)
The Human Apoptosis Array (43 targets) from AssayGenie (SARB0021) is a powerful tool for researchers studying the intricate mechanisms of apoptosis in human samples. This array allows for the simultaneous detection and quantification of 43 key proteins involved in the apoptotic process, providing valuable insights into cell death pathways.With a focus on high sensitivity and specificity, this apoptosis array offers reliable and reproducible results for a wide range of sample types, including cell lysates, tissue lysates, and serum. The array is designed for use in various applications, such as protein profiling, biomarker discovery, and drug development, making it an essential tool for advancing research in fields such as cancer biology, immunology, and drug discovery.
By leveraging the comprehensive information provided by the Human Apoptosis Array (43 targets), researchers can gain a deeper understanding of the molecular events that govern apoptosis, ultimately leading to the development of novel therapeutic strategies for targeting cell death pathways in various disease states. Unlock the secrets of apoptosis with the cutting-edge technology of the Human Apoptosis Array from AssayGenie.
| Product Sku: | SARB0021 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 43 |
| Gene Symbols: | BAD, BAX, BCL2, BCL2L11, BCL2L2, BID, BIRC3, BIRC5, BIRC7, CASP3|CASP8, CD40, CD40LG, CDKN1A, CYCS, DIABLO, FAS, FASLG, HSPA14, HSPA4, HSPB1, HTRA2, IGF1, IGF1R, IGF2, IGFBP1, IGFBP2, IGFBP3, IGFBP4, IGFBP5, IGFBP6, LTA, SSSCA1, TNF, TNFRSF10A, TNFRSF10B, TNFRSF10C, TNFRSF10D, TNFRSF1A, TNFRSF1B, TNFRSF21, TP53, XIAP |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | Akt Signaling / IGF Signaling / p53 Signaling |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
bad
| bax
| bcl-2
| bcl-w
| BID
|
BIM
| Caspase-3
| Caspase-8
| CD40 (TNFRSF5)
| CD40 Ligand (TNFSF5)
|
cIAP-2
| Cytochrome C
| DR6 (TNFRSF21)
| Fas (TNFRSF6/Apo-1)
| Fas Ligand (TNFSF6)
|
HSP27
| HSP60
| HSP70
| HTRA2
| IGF-1
|
IGF-2
| IGFBP-1
| IGFBP-2
| IGFBP-3
| IGFBP-4
|
IGFBP-5
| IGFBP-6
| IGF-1 R
| livin
| p21
|
p27
| p53
| SMAC
| Survivin (BIRC5)
| TNF RI (TNFRSF1A)
|
TNF RII (TNFRSF1B)
| TNF alpha
| TNF beta (TNFSF1B)
| TRAIL R1 (TNFRSF10A/DR4)
| TRAIL R2 (TNFRSF10B/DR5)
|
TRAIL R3 (TNFRSF10C)
| TRAIL R4 (TNFRSF10D)
| XIAP
| ||
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human Apoptosis Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- Protease Inhibitor Cocktail
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
