The Human Col3 (Collagen Type 3) CLIA Kit is a high-quality assay designed for the precise measurement of collagen type 3 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers unmatched sensitivity and specificity, ensuring accurate and reproducible results for various research applications.Collagen type 3 is a crucial component of connective tissues, providing structural support and elasticity. Abnormal levels of collagen type 3 have been linked to various health conditions, including cardiovascular diseases, fibrosis, and connective tissue disorders.
As such, the Human Col3 CLIA Kit serves as a valuable tool for studying these conditions and developing potential therapeutic interventions.Overall, the Human Col3 CLIA Kit from Assay Genie provides researchers with a reliable and efficient solution for investigating the role of collagen type 3 in health and disease.
Product Name:
Human COL3 (Collagen Type 3) CLIA Kit
SKU:
HUES00484
Target:
Human COL3 (Collagen Type 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human COL3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human COL3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human COL3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human COL3. You can calculate the concentration of Human COL3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-114
96-111
101-115
Average (%)
105
102
108
1:4
Range (%)
89-104
95-109
104-117
Average (%)
97
103
110
1:8
Range (%)
97-115
86-99
100-115
Average (%)
105
92
105
1:16
Range (%)
100-114
95-108
87-100
Average (%)
107
102
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-106
97
EDTA plasma (n=5)
100-116
107
Cell culture media (n=5)
96-108
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
368.48
925.09
2838.56
345.84
837.03
3109.31
Standard deviation
34.23
92.23
226.52
40.71
77.76
339.23
C V (%)
9.29
9.97
7.98
11.77
9.29
10.91
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human COL3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human COL3 in samples. No significant cross-reactivity or interference between Human COL3 and analogues was observed.
