Description
Human Stem Cell Array (15 targets) (SARB0066)
The Human Stem Cell Array (SARB0066) offered by AssayGenie is a comprehensive tool for researchers studying stem cell biology. This array contains 15 unique targets related to stem cell markers, transcription factors, and differentiation markers, allowing for a detailed analysis of gene expression profiles in stem cells. Each target on the array is carefully selected to provide valuable insights into the pluripotency, self-renewal, and differentiation potential of stem cells. By using this array, researchers can identify key genes involved in stem cell maintenance and differentiation, facilitating the development of novel therapies and regenerative medicine approaches.
The Human Stem Cell Array is optimized for use with human samples and is compatible with various detection methods such as qPCR and microarray analysis. With its high sensitivity and specificity, this array offers a powerful tool for uncovering the molecular mechanisms underlying stem cell function and regulation. Whether studying embryonic stem cells, induced pluripotent stem cells, or tissue-specific stem cells, the Human Stem Cell Array is an indispensable resource for advancing stem cell research.
| Product Sku: | SARB0066 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 15 |
| Gene Symbols: | AFP, BMPR1A, BMPR1B, CD38, CDH1, CGB, GATA4, KDR, NANOG, NES|PDX1, POU5F1, SOX17, SOX2, T |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection Detection of Relative Protein Expression Detecting Patterns of Cytokine Expression Biomarker/ Key Factor Screening Identifying Key Factors Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
Alpha-fetoprotein
| BMPR-IA (ALK-3)
| ALK-6 (BMPR-IB)
| Brachyury (Protein T)
| CD38
|
E-Cadherin
| GATA4
| hCG beta
| Nanog
| Nestin
|
OCT-4
| PDX-1 (IPF1)
| SOX17
| SOX2
| VEGFR2
|
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human Stem Cell Array C1 Membranes(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
