Human TNF- alpha (Tumor Necrosis Factor Alpha) CLIA Kit
The Human TNF-Alpha (Tumor Necrosis Factor Alpha) CLIA Kit is a cutting-edge diagnostic tool designed for the accurate and precise detection of TNF-Alpha levels in human samples, including serum, plasma, and cell culture supernatants. This innovative kit offers exceptional sensitivity and specificity, ensuring reliable and consistent results for a wide range of research applications.TNF-Alpha is a key inflammatory cytokine with a vital role in immune response regulation, cell differentiation, and apoptosis. Dysregulation of TNF-Alpha has been linked to various debilitating conditions such as autoimmune diseases, inflammatory disorders, and cancer.
As such, the Human TNF-Alpha CLIA Kit serves as an indispensable tool for studying TNF-Alpha biology, disease pathogenesis, and therapeutic interventions.With its advanced technology and reliable performance, the Human TNF-Alpha CLIA Kit from Assaygenie is a valuable asset for researchers and clinicians seeking to unravel the complexities of TNF-Alpha biology and its implications for human health.
Product Name:
Human TNF- alpha (Tumor Necrosis Factor Alpha) CLIA Kit
SKU:
HUES00105
Target:
Human TNF- alpha (Tumor Necrosis Factor Alpha)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TNF-α. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TNF-α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TNF-α, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TNF-α. You can calculate the concentration of Human TNF-α in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-99
98-112
97-110
Average (%)
93
105
103
1:4
Range (%)
99-111
101-117
85-98
Average (%)
105
109
90
1:8
Range (%)
90-102
91-105
93-106
Average (%)
97
99
98
1:16
Range (%)
103-121
85-96
103-116
Average (%)
110
91
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-113
107
EDTA plasma (n=5)
92-103
98
Cell culture media (n=5)
87-101
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.6
58.03
169.01
27.58
54.45
158.5
Standard deviation
2.69
5.5
13.54
2.46
6.4
14.99
C V (%)
10.51
9.48
8.01
8.92
11.75
9.46
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TNF-α concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TNF-α in samples. No significant cross-reactivity or interference between Human TNF-α and analogues was observed.
