The Mouse TNF-alpha (Tumor Necrosis Factor-alpha) CLIA Kit is a highly sensitive and specific assay designed for the accurate quantification of TNF-alpha levels in mouse samples. This kit is ideal for researchers studying inflammation, immune responses, and various disease models where TNF-alpha plays a critical role.TNF-alpha is a key cytokine involved in inflammatory responses, immune regulation, and cell death pathways. It is implicated in various diseases such as rheumatoid arthritis, inflammatory bowel disease, and sepsis, making it a valuable biomarker for both basic research and clinical studies. With its high sensitivity and reproducibility, the Mouse TNF-alpha CLIA Kit offers reliable and consistent results, enabling researchers to confidently analyze TNF-alpha expression levels in a variety of sample types including serum, plasma, and tissue culture supernatants. This kit is a powerful tool for advancing our understanding of TNF-alpha biology and developing potential therapeutic strategies targeting this important cytokine.
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.
