Description
KLF Transcription Factor Activity Assay
The KLF Transcription Factor Activity Assay Kit is a powerful tool for studying the activity of KLF transcription factors in various biological samples. This kit offers high sensitivity and specificity, allowing for accurate and reproducible results in applications such as drug discovery, cancer research, and cell signaling studies.KLF transcription factors play a key role in regulating gene expression and are involved in diverse cellular processes such as cell proliferation, differentiation, and apoptosis. Dysregulation of KLF activity has been linked to various diseases including cancer, cardiovascular disorders, and inflammatory conditions, making this assay kit essential for understanding the mechanisms underlying these pathologies.
With easy-to-follow protocols and a user-friendly format, the KLF Transcription Factor Activity Assay Kit is suitable for both experienced researchers and beginners in the field. Trust Assay Genie for top-quality research tools that deliver reliable data for your scientific investigations.
| Product Name: | KLF Transcription Factor Activity Assay |
| Product Code: | TFAB00193 |
| Target: | KLF |
| Synonyms: | Erythroid krueppel-like transcription factor, EKLF |
| Reactivity: | Human, Mouse, Rat |
| Sample Types: | Nuclear or cell lysates |
The Assay Genie KLF transcription factor activity assay allows for the detection and qualitative analysis of endogenous levels of activated transcription factors in a variety of nuclear and cell lysates
Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
| Assay Time: | 4.5 hours |
| Detection Method: | Colorimetric 450 nm |
| Size: | 12 x 8-Well Microstrips |
| Storage: | 4°C for 6 months |
| UniProt Protein Function: | KLF1: Transcription regulator of erythrocyte development that probably serves as a general switch factor during erythropoiesis. Is a dual regulator of fetal-to-adult globin switching. Binds to the CACCC box in the beta-globin gene promoter and acts as a preferential activator of this gene. Furthermore, it binds to the BCL11A promoter and activates expression of BCL11A, which in turn represses the HBG1 and HBG2 genes. This dual activity ensures that, in most adults, fetal hemoglobin levels are low. Able to activate CD44 and AQP1 promoters. When sumoylated, acts as a transcriptional repressor by promoting interaction with CDH2/MI2beta and also represses megakaryocytic differentiation. Defects in KLF1 are the cause of congenital dyserythropoietic anemia type 4 (CDA4). It is a blood disorder characterized by ineffective erythropoiesis and hemolysis resulting in anemia. Circulating erythroblasts and erythroblasts in the bone marrow show various morphologic abnormalities. Affected individuals with CDA4 also have increased levels of fetal hemoglobin. Belongs to the krueppel C2H2-type zinc-finger protein family. |
| UniProt Protein Details: | Protein type:C2H2-type zinc finger protein; DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 19p13.13 Cellular Component: nuclear chromatin; nucleus Molecular Function:transcription factor activity Biological Process: erythrocyte differentiation; positive regulation of transcription, DNA-dependent; regulation of transcription, DNA-dependent Disease: Anemia, Congenital Dyserythropoietic, Type Iv; Blood Group--lutheran Inhibitor; Fetal Hemoglobin Quantitative Trait Locus 6 |
| NCBI Summary: | This gene encodes a hematopoietic-specific transcription factor that induces high-level expression of adult beta-globin and other erythroid genes. The zinc-finger protein binds to the DNA sequence CCACACCCT found in the beta hemoglobin promoter. Heterozygous loss-of-function mutations in this gene result in the dominant In(Lu) blood phenotype. [provided by RefSeq, Oct 2009] |
| UniProt Code: | Q13351 |
| NCBI GenInfo Identifier: | 2501699 |
| NCBI Gene ID: | 10661 |
| NCBI Accession: | Q13351.1 |
| UniProt Secondary Accession: | Q13351,Q6PIJ5, Q92899, |
| UniProt Related Accession: | Q13351 |
| Molecular Weight: | 38kDa |
| NCBI Full Name: | Krueppel-like factor 1 |
| NCBI Synonym Full Names: | Kruppel like factor 1 |
| NCBI Official Symbol: | KLF1 |
| NCBI Official Synonym Symbols: | EKLF |
| NCBI Protein Information: | Krueppel-like factor 1 |
| UniProt Protein Name: | Krueppel-like factor 1 |
| UniProt Synonym Protein Names: | Erythroid krueppel-like transcription factor; EKLF |
| Protein Family: | Krueppel-like factor |
| UniProt Gene Name: | KLF1 |
