The [KO Validated] ARHGEF1 Antibody (CAB4274) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is produced in rabbits and has high reactivity with human samples, making it suitable for use in Western blot applications. By binding specifically to the ARHGEF1 protein, this antibody allows for the visualization and analysis of ARHGEF1 expression in various cell types.ARHGEF1, also known as Rho guanine nucleotide exchange factor 1, is involved in the activation of Rho GTPases, which play essential roles in cytoskeletal organization and cell motility.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
[KO Validated] ARHGEF1 Antibody
SKU:
CAB4274
Size:
20μL, 100μL
Reactivity:
Human, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
LSC, GEF1, IMD62, LBCL2, SUB1.5, P115-RHOGEF, F1
Positive Sample:
HeLa, Jurkat, LO2
Cellular Localization:
Cytoplasm, Membrane.
Calculated MW:
102kDa
Observed MW:
115kDa
Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined.
Purification Method
Affinity purification
Gene ID
9138
RRID
AB_2863222
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using [KO Validated] ARHGEF1 Rabbit pAb (CAB4274) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Western blot analysis of lysates from wild type (WT) and ARHGEF1 knockout (KO) 293T cells, using [KO Validated] ARHGEF1 Rabbit pAb (CAB4274) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
