The [KO Validated] Bcl10 Antibody (CAB1106) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the BCL10 protein, researchers can accurately detect and analyze its expression in various cell types.BCL10 is a key player in immune signaling pathways, regulating processes such as cell survival and inflammation. Dysregulation of BCL10 has been linked to various diseases, including lymphoma and inflammatory disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
[KO Validated] Bcl10 Antibody
SKU:
CAB1106
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CLAP, mE10, CIPER, IMD37, c-E10, CARMEN, 10
Positive Sample:
Raji, Mouse spleen, Rat spleen
Cellular Localization:
Cytoplasm, Membrane Raft, Perinuclear Region.
Calculated MW:
26kDa
Observed MW:
30kDa
This gene was identified by its translocation in a case of mucosa-associated lymphoid tissue (MALT) lymphoma. The protein encoded by this gene contains a caspase recruitment domain (CARD), and has been shown to induce apoptosis and to activate NF-kappaB. This protein is reported to interact with other CARD domain containing proteins including CARD9, 10, 11 and 14, which are thought to function as upstream regulators in NF-kappaB signaling. This protein is found to form a complex with MALT1, a protein encoded by another gene known to be translocated in MALT lymphoma. MALT1 and this protein are thought to synergize in the activation of NF-kappaB, and the deregulation of either of them may contribute to the same pathogenetic process that leads to the malignancy. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
8915
RRID
AB_2861498
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type (WT) and Bcl10 knockout (KO) HeLa cells, using [KO Validated] Bcl10 Rabbit pAb (CAB1106) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
