Anti-Cyclin D3 Antibody (CAB0746)

Antibody Name:	Anti-Cyclin D3 Antibody [KO Validated]
Antibody SKU:	CAB0746
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 201-292 of human Cyclin D3 (NP_001751.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	HeLa, K-562, Mouse spleen

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 201-292 of human Cyclin D3 (NP_001751.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SMIA TGSI GAAV QGLG ACSM SGDE LTEL LAGI TGTE VDCL RACQ EQIE AALR ESLR EASQ TSSS PAPK APRG SSSQ GPSQ TSTP TDVT AIHL
Gene ID:	896
Uniprot:	P30281
Cellular Location:	Cytoplasm, Membrane, Nucleus
Calculated MW:	9kDa/22kDa/24kDa/32kDa
Observed MW:	36kDa

Synonyms:

CCND3, cyclin D3, Cyclin D3

Background:

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation. Several transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	CCND3: Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/p27Kip1, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Interacts with the CDK4 and CDK6 protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Interacts with ATF5. Interacts with EIF3K. Component of the ternary complex cyclin D/CDK4/p27Kip1 required for nuclear translocation and modulation of CDK4-mediated kinase activity. Can form similar complexes with either p21Cip1 or CDKN2A. Belongs to the cyclin family. Cyclin D subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Nuclear receptor co-regulator; Cell cycle regulation; Activator Chromosomal Location of Human Ortholog: 6p21 Cellular Component: cyclin-dependent protein kinase holoenzyme complex; cytoplasm; focal adhesion; nucleoplasm; plasma membrane Molecular Function:protein binding; protein kinase binding Biological Process: positive regulation of cyclin-dependent protein kinase activity; positive regulation of protein amino acid phosphorylation
NCBI Summary:	The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]
UniProt Code:	P30281
NCBI GenInfo Identifier:	20981685
NCBI Gene ID:	896
NCBI Accession:	P30281.2
UniProt Secondary Accession:	P30281,Q5T8J0, Q6FG62, Q96F49, B2RD63, B3KQ22, E9PAS4 E9PB36,
UniProt Related Accession:	P30281
Molecular Weight:	9,736 Da
NCBI Full Name:	G1/S-specific cyclin-D3
NCBI Synonym Full Names:	cyclin D3
NCBI Official Symbol:	CCND3
NCBI Protein Information:	G1/S-specific cyclin-D3
UniProt Protein Name:	G1/S-specific cyclin-D3
Protein Family:	G1/S-specific cyclin
UniProt Gene Name:	CCND3
UniProt Entry Name:	CCND3_HUMAN

	Western blot analysis of extracts of various cell lines, using Cyclin D3 antibody (CAB0746) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Western blot analysis of extracts from normal (control) and Cyclin D3 knockout (KO) HeLa cells, using Cyclin D3 antibody (CAB0746) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies	ELISA Kits
Anti-Cyclin D3 Antibody (CAB1084)	Human G1/S-specific cyclin-D3 (CCND3) ELISA Kit
Anti-Cyclin D3 Antibody (CAB3989)	Cyclin D3 Colorimetric Cell-Based ELISA Kit
CCND3 Antibody (PACO03169)	Cyclin D3 (Phospho-Thr283) Colorimetric Cell-Based ELISA Kit
Phospho-CCND3 (T283) Antibody (PACO05650)
CCND3 Antibody (PACO08247)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)