Anti-Cyclin B1 Antibody (CAB19037)

Antibody Name:	Anti-Cyclin B1 Antibody [KO Validated]
Antibody SKU:	CAB19037
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	A synthesized peptide derived from human Cyclin B1

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	Jurkat, Mouse testis, HeLa

Immunogen:	A synthesized peptide derived from human Cyclin B1
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	891
Uniprot:	P14635
Cellular Location:	Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center
Calculated MW:	55kDa
Observed MW:	55kDa

	Western blot analysis of extracts from normal (control) and Cyclin B1 knockout (KO) HeLa cells, using Cyclin B1 antibody (CAB19037) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min.
	Western blot analysis of extracts of various cell lines, using Cyclin B1 antibody (CAB19037) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min.

Synonyms:

CCNB, CCNB1, Cyclin B1, cyclin B1

Background:

The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008]

UniProt Protein Function:	CCNB1: a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Complexes with cdc2 to form the maturation-promoting factor (MPF). Two alternatively spliced isoforms have been found, a constitutively expressed form and a cell cycle-regulated form that is expressed predominantly during G2/M phase.
UniProt Protein Details:	Protein type:Cell cycle regulation; Activator Chromosomal Location of Human Ortholog: 5q12 Cellular Component: nucleoplasm; spindle pole; centrosome; membrane; cytoplasm; nucleus; cytosol Molecular Function:protein binding; patched binding; histone kinase activity; protein kinase binding Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of histone phosphorylation; regulation of cell cycle; ventricular cardiac muscle cell development; positive regulation of attachment of spindle microtubules to kinetochore; mitotic nuclear envelope disassembly; positive regulation of mRNA 3'-end processing; positive regulation of mitotic cell cycle; positive regulation of fibroblast proliferation; negative regulation of protein amino acid phosphorylation; tissue regeneration; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; protein complex assembly; G2/M transition of mitotic cell cycle; positive regulation of cardiac muscle cell proliferation; response to drug; in utero embryonic development; oocyte maturation; mitotic metaphase plate congression; response to DDT; gut development; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; response to mechanical stimulus; cell division; spermatogenesis; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; mitotic spindle stabilization; G1/S transition of mitotic cell cycle
NCBI Summary:	The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008]
UniProt Code:	P14635
NCBI GenInfo Identifier:	116176
NCBI Gene ID:	891
NCBI Accession:	P14635.1
UniProt Secondary Accession:	P14635,Q5TZP9, A8K066,
UniProt Related Accession:	P14635
Molecular Weight:	44,102 Da
NCBI Full Name:	G2/mitotic-specific cyclin-B1
NCBI Synonym Full Names:	cyclin B1
NCBI Official Symbol:	CCNB1
NCBI Official Synonym Symbols:	CCNB
NCBI Protein Information:	G2/mitotic-specific cyclin-B1; G2/mitotic-specific cyclin B1
UniProt Protein Name:	G2/mitotic-specific cyclin-B1
UniProt Gene Name:	CCNB1
UniProt Entry Name:	CCNB1_HUMAN

Antibodies	ELISA Kits
Anti-Cyclin B1 Antibody (CAB16038)	Human CCNB1 / Cyclin B1 ELISA Kit
Anti-Cyclin B1 Antibody (CAB2056)	Human CCNB (Cyclin-B) CLIA Kit (HUES00214)
Anti-Cyclin B1 Antibody (CAB16800)	Human CCNB (Cyclin-B) ELISA Kit (HUES01523)
Phospho-CCNB1 (S126) Antibody (PACO00198)	Cyclin B1 Colorimetric Cell-Based ELISA Kit
Phospho-CCNB1 (S147) Antibody (PACO05644)	Cyclin B1 (Phospho-Ser126) Colorimetric Cell-Based ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Mouse IgG (H+L) Antibody (CABS003)