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[KO Validated] CSPG4 Rabbit Polyclonal Antibody (CAB3592)

SKU:
CAB3592
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cardiovascular
  • KO Validated Antibodies 1 Anti-CSPG4 Antibody CAB3592KO Validated
  • KO Validated Antibodies 1 Anti-CSPG4 Antibody CAB3592KO Validated
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:[KO Validated] CSPG4 Rabbit Polyclonal Antibody
SKU:CAB3592
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1340-1563 of human NG2/CSPG4 (NP_001888.2).
Sequence:LGAP LEGV LVEL EVLP AAIP LEAQ NFSV PEGG SLTL APPL LRVS GPYF PTLL GLSL QVLE PPQH GALQ KEDG PQAR TLSA FSWR MVEE QLIR YVHD GSET LTDS FVLM ANAS EMDR QSHP VAFT VTVL PVND QPPI LTTN TGLQ MWEG ATAP IPAE ALRS TDGD SGSE DLVY TIEQ PSNG RVVL RGAP GTEV RSFT QAQL DGGL VLFS HRGT LDGG FRFR LSDG
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200
Synonyms:NG2; MCSP; MCSPG; MSK16; CSPG4A; HMW-MAA; MEL-CSPG; G4
Positive Sample:HeLa,A375,Mouse heart,Rat brain,Rat heart
Conjugate:Unconjugated
Cellular Localization:Apical cell membrane, Cell projection, Extracellular side, Single-pass type I membrane protein, lamellipodium membrane.
Calculated MW:251kDa
Observed MW:250kDa-450kDa

A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.

Purification Method:Affinity purification
Gene ID:1464
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from A375 cells, using [KO Validated] NG2/CSPG4 Rabbit pAb (CAB3592) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

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