The [KO Validated] DDIT4 Antibody (CAB8086) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced against DDIT4 in rabbits, is specifically validated for knockout (KO) studies and demonstrated to have high specificity and sensitivity in Western blot applications.DDIT4, also known as REDD1, is a critical factor in the cellular response to nutrient deprivation, hypoxia, and other stress conditions. By binding to DDIT4, this polyclonal antibody allows for precise detection and quantification of DDIT4 expression in various cell types, enabling researchers to explore its role in cellular metabolism, signal transduction, and disease processes.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
[KO Validated] DDIT4 Antibody
SKU:
CAB8086
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
Dig2, REDD1, REDD-1, T4
Positive Sample:
A-549, DU145, U-251MG
Cellular Localization:
Cytoplasm, Mitochondrion, Cytosol.
Calculated MW:
25kDa
Observed MW:
32kDa
Predicted to enable 14-3-3 protein binding activity. Involved in defense response to virus; negative regulation of TOR signaling; and response to hypoxia. Located in cytosol.
Purification Method
Affinity purification
Gene ID
54541
RRID
AB_2863566
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type (WT) and DDIT4 knockout (KO) HeLa cells, using [KO Validated] DDIT4 Rabbit pAb (CAB8086) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
Western blot analysis of various lysates using [KO Validated] DDIT4 Rabbit pAb (CAB8086) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
