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[KO Validated] DDIT4 Rabbit Polyclonal Antibody (CAB8086)

SKU:
CAB8086
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology
  • KO Validated Antibodies 1 Anti-DDIT4 Antibody CAB8086KO Validated
  • KO Validated Antibodies 1 Anti-DDIT4 Antibody CAB8086KO Validated
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:[KO Validated] DDIT4 Rabbit Polyclonal Antibody
SKU:CAB8086
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-232 of human DDIT4 (NP_061931.1).
Sequence:MPSL WDRF SSSS TSSS PSSL PRTP TPDR PPRS AWGS ATRE EGFD RSTS LESS DCES LDSS NSGF GPEE DTAY LDGV SLPD FELL SDPE DEHL CANL MQLL QESL AQAR LGSR RPAR LLMP SQLV SQVG KELL RLAY SEPC GLRG ALLD VCVE QGKS CHSV GQLA LDPS LVPT FQLT LVLR LDSR LWPK IQGL FSSA NSPF LPGF SQSL TLST GFRV IKKK LYSS EQLL IEEC
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:Dig2; REDD1; REDD-1; T4
Positive Sample:A-549,DU145,U-251MG
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Mitochondrion, cytosol.
Calculated MW:25kDa
Observed MW:32kDa

Predicted to enable 14-3-3 protein binding activity. Involved in defense response to virus; negative regulation of TOR signaling; and response to hypoxia. Located in cytosol.

Purification Method:Affinity purification
Gene ID:54541
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts from normal (control) and DDIT4 knockout (KO) HeLa cells, using DDIT4 antibody (CAB8086) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.

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