Anti-ERP29 Antibody (CAB7959)

Antibody Name:	[KO Validated] ERP29 Rabbit Polyclonal Antibody
Antibody SKU:	CAB7959
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide of human ERP29

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	HL-60, THP-1, HeLa, 293T, Mouse testis, Mouse pancreas, Mouse thymus, Mouse spleen

Immunogen:	A synthetic peptide of human ERP29
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	10961
Uniprot:	P30040
Cellular Location:	Endoplasmic reticulum lumen, Melanosome
Calculated MW:	5kDa/28kDa
Observed MW:	29kDa

Synonyms:

ERP29, C12orf8, ERp28, ERp31, HEL-S-107, PDI-DB, PDIA9

Background:

This gene encodes a protein which localizes to the lumen of the endoplasmic reticulum (ER). It is a member of the protein disulfide isomerase (PDI) protein family but lacks an active thioredoxin motif, suggesting that this protein does not function as a disulfide isomerase. The canonical protein dimerizes and is thought to play a role in the processing of secretory proteins within the ER. Alternative splicing results in multiple transcript variants encoding different isoforms.

UniProt Protein Function:	ERP29: Does not seem to be a disulfide isomerase. Plays an important role in the processing of secretory proteins within the endoplasmic reticulum (ER), possibly by participating in the folding of proteins in the ER.
UniProt Protein Details:	Protein type:Chaperone Chromosomal Location of Human Ortholog: 12q24.13 Cellular Component: cell surface; endoplasmic reticulum; membrane; smooth endoplasmic reticulum; transport vesicle Molecular Function:chaperone binding; protein disulfide isomerase activity; protein homodimerization activity Biological Process: activation of MAPK activity; intracellular protein transport; negative regulation of protein secretion; positive regulation of protein amino acid phosphorylation
NCBI Summary:	This gene encodes a reticuloplasmin, a protein which resides in the lumen of the endoplasmic reticulum (ER). The protein shows sequence similarity to the protein disulfide isomerase family. However, it lacks the thioredoxin motif characteristic of this family, suggesting that this protein does not function as a disulfide isomerase. The protein dimerizes and is thought to play a role in the processing of secretory proteins within the ER. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
UniProt Code:	P30040
NCBI GenInfo Identifier:	6015110
NCBI Gene ID:	10961
NCBI Accession:	P30040.4
UniProt Secondary Accession:	P30040,Q3MJC3, Q6FHT4, C9J183,
UniProt Related Accession:	P30040
Molecular Weight:	5,477 Da
NCBI Full Name:	Endoplasmic reticulum resident protein 29
NCBI Synonym Full Names:	endoplasmic reticulum protein 29
NCBI Official Symbol:	ERP29
NCBI Official Synonym Symbols:	ERp28; ERp31; PDIA9; PDI-DB; C12orf8; HEL-S-107
NCBI Protein Information:	endoplasmic reticulum resident protein 29
UniProt Protein Name:	Endoplasmic reticulum resident protein 29
UniProt Synonym Protein Names:	Endoplasmic reticulum resident protein 28; ERp28; Endoplasmic reticulum resident protein 31; ERp31
Protein Family:	Endoplasmic reticulum resident protein
UniProt Gene Name:	ERP29
UniProt Entry Name:	ERP29_HUMAN

	Western blot analysis of extracts of various cell lines, using ERP29 antibody (CAB7959) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Western blot analysis of extracts from normal (control) and ERP29 knockout (KO) 293T cells, using ERP29 antibody (CAB7959) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)