The [KO Validated] Glutathione Synthetase (GSS) Antibody (CAB14535) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, derived from rabbit sources, has high specificity for GSS in human samples and has been validated for Western blot applications.GSS, also known as glutathione synthetase, plays a crucial role in the synthesis of the antioxidant glutathione, which protects cells from oxidative stress and maintains cellular homeostasis. Dysregulation of GSS activity has been implicated in diseases such as cancer, neurodegenerative disorders, and metabolic diseases, making it an important target for therapeutic intervention.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
GSHS, HEL-S-64p, HEL-S-88n, S)
Positive Sample:
293T, SH-SY5Y, Mouse liver, Mouse kidney, Rat lung
Cellular Localization:
Cytosol, Extracellular Exosome.
Calculated MW:
52kDa
Observed MW:
52kDa
Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency.
Purification Method
Affinity purification
Gene ID
2937
RRID
AB_2861700
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and Glutathione Synthetase (Glutathione Synthetase (GSS)) knockout (KO) 293T cells, using [KO Validated] Glutathione Synthetase (GSS) Rabbit pAb (CAB14535) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Immunofluorescence analysis of NIH/3T3 cells using Glutathione Synthetase (GSS) Rabbit pAb (CAB14535) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using Glutathione Synthetase (GSS) Rabbit pAb (CAB14535) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] Glutathione Synthetase (GSS) Rabbit pAb (CAB14535) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using [KO Validated] Glutathione Synthetase (GSS) Rabbit pAb (CAB14535) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
