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[KO Validated] HMGB1 Rabbit Monoclonal Antibody (CAB19529)

SKU:
CAB19529
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology
  • KO Validated Antibodies 2 Anti-HMGB1 Antibody KO Validated CAB19529
  • KO Validated Antibodies 2 Anti-HMGB1 Antibody KO Validated CAB19529
  • KO Validated Antibodies 2 Anti-HMGB1 Antibody KO Validated CAB19529
  • KO Validated Antibodies 2 Anti-HMGB1 Antibody KO Validated CAB19529
  • KO Validated Antibodies 2 Anti-HMGB1 Antibody KO Validated CAB19529
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:[KO Validated] HMGB1 Rabbit Monoclonal Antibody
SKU:CAB19529
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 100-200 of human HMGB1 (P09429).
Sequence:SAFF LFCS EYRP KIKG EHPG LSIG DVAK KLGE MWNN TAAD DKQP YEKK AAKL KEKY EKDI AAYR AKGK PDAA KKGV VKAE KSKK KKEE EEDE EDEE DEEE E
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:2000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200
Synonyms:HMG1; HMG3; HMG-1; SBP-1; B1
Positive Sample:293T,HeLa,Jurkat,Mouse liver,Mouse brain,Mouse testis,Rat liver
Conjugate:Unconjugated
Cellular Localization:Cell membrane, Chromosome, Cytoplasm, Endoplasmic reticulum-Golgi intermediate compartment, Endosome, Extracellular side, Nucleus, Peripheral membrane protein, Secreted.
Calculated MW:25kDa
Observed MW:25kDa

This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein.

Purification Method:Affinity purification
Gene ID:3146
Clone Number:ARC0001
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of various cell lines, using HMGB1 antibody (CAB19529) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.

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