Anti-HuR/ELAVL1 Antibody (CAB19622)

Antibody Name:	Anti-HuR / ELAVL1 Antibody [KO Validated]
Antibody SKU:	CAB19622
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthesized peptide derived from human HuR / ELAVL1.

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, HeLa, Jurkat, Mouse brain, Mouse spleen, Mouse thymus, Rat brain

Immunogen:	A synthesized peptide derived from human HuR / ELAVL1.
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	1994
Uniprot:	Q15717
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	36kDa
Observed MW:	36KDa

	Western blot analysis of extracts from normal (control) and HuR / ELAVL1 knockout (KO) 293T cells, using HuR / ELAVL1 antibody (CAB19622) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Western blot analysis of extracts of various cell lines, using HuR / ELAVL1 antibody (CAB19622) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded rat liver using HuR / ELAVL1 antibody (CAB19622) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophageal using HuR / ELAVL1 antibody (CAB19622) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse spleen using HuR / ELAVL1 antibody (CAB19622) at dilution of 1:100 (40x lens).

Synonyms:

ELAV1, HUR, Hua, MelG, HuR / ELAVL1, ELAVL1

Background:

The protein encoded by this gene is a member of the ELAVL family of RNA-binding proteins that contain several RNA recognition motifs, and selectively bind AU-rich elements (AREs) found in the 3' untranslated regions of mRNAs. AREs signal degradation of mRNAs as a means to regulate gene expression, thus by binding AREs, the ELAVL family of proteins play a role in stabilizing ARE-containing mRNAs. This gene has been implicated in a variety of biological processes and has been linked to a number of diseases, including cancer. It is highly expressed in many cancers, and could be potentially useful in cancer diagnosis, prognosis, and therapy. [provided by RefSeq, Sep 2012]

UniProt Protein Function:	HuR: Involved in 3'-UTR ARE-mediated MYC stabilization. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, HUR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro. Identified in a mRNP complex, at least composed of DHX9, DDX3X, ELAVL1, HNRNPU, IGF2BP1, ILF3, PABPC1, PCBP2, PTBP2, STAU1, STAU2, SYNCRIP and YBX1. Interacts with ANP32A, EIF2C1 and EIF2C2. Ubiquitous. Belongs to the RRM elav family.
UniProt Protein Details:	Protein type:RNA-binding Chromosomal Location of Human Ortholog: 19p13.2 Cellular Component: nucleoplasm; membrane; cytoplasm; cytosol; nucleus Molecular Function:mRNA binding; protein binding; mRNA 3'-UTR binding; RNA binding; double-stranded RNA binding; nucleotide binding; AU-rich element binding; protein kinase binding Biological Process: positive regulation of translation; multicellular organismal development; mRNA stabilization; gene expression
NCBI Summary:	The protein encoded by this gene is a member of the ELAVL family of RNA-binding proteins that contain several RNA recognition motifs, and selectively bind AU-rich elements (AREs) found in the 3' untranslated regions of mRNAs. AREs signal degradation of mRNAs as a means to regulate gene expression, thus by binding AREs, the ELAVL family of proteins play a role in stabilizing ARE-containing mRNAs. This gene has been implicated in a variety of biological processes and has been linked to a number of diseases, including cancer. It is highly expressed in many cancers, and could be potentially useful in cancer diagnosis, prognosis, and therapy. [provided by RefSeq, Sep 2012]
UniProt Code:	Q15717
NCBI GenInfo Identifier:	20981691
NCBI Gene ID:	1994
NCBI Accession:	Q15717.2
UniProt Related Accession:	Q15717
Molecular Weight:
NCBI Full Name:	ELAV-like protein 1
NCBI Synonym Full Names:	ELAV like RNA binding protein 1
NCBI Official Symbol:	ELAVL1
NCBI Official Synonym Symbols:	HUR; Hua; MelG; ELAV1
NCBI Protein Information:	ELAV-like protein 1
UniProt Protein Name:	ELAV-like protein 1
UniProt Synonym Protein Names:	Hu-antigen R; HuR
UniProt Gene Name:	ELAVL1
UniProt Entry Name:	ELAV1_HUMAN

Antibodies

Anti-HuR / ELAVL1 Antibody (CAB1608)[KO Validated]

Anti-HuR / ELAVL1 Antibody (CAB6089)[KO Validated]

Secondary Antibody

Anti-HRP Goat Anti-Mouse IgG (H+L) Antibody (CABS003)