The [KO Validated] RALBP1 Antibody (CAB1140) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, has high specificity and sensitivity for detecting RALBP1 in various tissues and cell types, making it an ideal choice for Western blot and immunohistochemistry applications.RALBP1, also known as RLIP76, is a multifunctional protein with roles in intracellular transport and lipid metabolism.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
[KO Validated] RALBP1 Antibody
SKU:
CAB1140
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RIP1, RLIP1, RLIP76, P1
Positive Sample:
A-673, Mouse spleen, Mouse lung
Cellular Localization:
Membrane, Peripheral Membrane Protein.
Calculated MW:
76kDa
Observed MW:
100kDa
RALBP1 plays a role in receptor-mediated endocytosis and is a downstream effector of the small GTP-binding protein RAL (see RALA; MIM 179550). Small G proteins, such as RAL, have GDP-bound inactive and GTP-bound active forms, which shift from the inactive to the active state through the action of RALGDS (MIM 601619), which in turn is activated by RAS (see HRAS; MIM 190020) (summary by Feig, 2003 [PubMed 12888294]).
Purification Method
Affinity purification
Gene ID
10928
RRID
AB_2861558
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using [KO Validated] RALBP1 Rabbit pAb (CAB1140) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of lysates from wild type (WT) and RALBP1 knockout (KO) HeLa cells, using [KO Validated] RALBP1 Rabbit pAb (CAB1140) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
