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[KO Validated] ROCK1 Rabbit Polyclonal Antibody (CAB1008)

SKU:
CAB1008
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death
  • KO Validated Antibodies 1 Anti-ROCK1 Antibody CAB1008KO Validated
  • KO Validated Antibodies 1 Anti-ROCK1 Antibody CAB1008KO Validated
  • KO Validated Antibodies 1 Anti-ROCK1 Antibody CAB1008KO Validated
  • KO Validated Antibodies 1 Anti-ROCK1 Antibody CAB1008KO Validated
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:[KO Validated] ROCK1 Rabbit Polyclonal Antibody
SKU:CAB1008
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 400-660 of human ROCK1 (NP_005397.1).
Sequence:YSNR RYLS SANP NDNR TSSN ADKS LQES LQKT IYKL EEQL HNEM QLKD EMEQ KCRT SNIK LDKI MKEL DEEG NQRR NLES TVSQ IEKE KMLL QHRI NEYQ RKAE QENE KRRN VENE VSTL KDQL EDLK KVSQ NSQL ANEK LSQL QKQL EEAN DLLR TESD TAVR LRKS HTEM SKSI SQLE SLNR ELQE RNRI LENS KSQT DKDY YQLQ AILE AERR DRGH DSEM IGDL QARI TSLQ EEVK HLKH NLEK VEGE RKEA QDML N
Tested Applications:WB IHC-P ELISA
Recommended Dilution:WB,1:500 - 1:1000 IHC-P,1:50 - 1:100
Synonyms:ROCK-I; P160ROCK; K1
Positive Sample:293T,U-251MG,Jurkat,BT-474
Conjugate:Unconjugated
Cellular Localization:Cell membrane, Cell projection, Cytoplasm, Golgi apparatus membrane, Peripheral membrane protein, bleb, centriole, centrosome, cytoskeleton, lamellipodium, microtubule organizing center, ruffle.
Calculated MW:158kDa
Observed MW:158kDa

This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18.

Purification Method:Affinity purification
Gene ID:6093
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates, using [KO Validated] ROCK1 Rabbit pAb (CAB1008) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

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