Anti-SMARCC1 Antibody (CAB6128)

Antibody Name:	Anti-SMARCC1 Antibody [KO Validated]
Antibody SKU:	CAB6128
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IP
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 80-320 of human SMARCC1 (NP_003065.3).

Application:	WB IHC IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:	Human
Positive Samples:	293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 80-320 of human SMARCC1 (NP_003065.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LVVQ LLQF QEDA FGKH VTNP AFTK LPAK CFMD FKAG GALC HILG AAYK YKNE QGWR RFDL QNPS RMDR NVEM FMNI EKTL VQNN CLTR PNIY LIPD IDLK LANK LKDI IKRH QGTF TDEK SKAS HHIY PYSS SQDD EEWL RPVM RKEK QVLV HWGF YPDS YDTW VHSN DVDA EIED PPIP EKPW KVHV KWIL DTDI FNEW MNEE DYEV DENR KPVS FRQR ISTK NEEP VRSP ERRD RKAS A
Gene ID:	6599
Uniprot:	Q92922
Cellular Location:	Nucleus
Calculated MW:	122kDa
Observed MW:	155KDa

Synonyms:

SMARCC1, BAF155, CRACC1, Rsc8, SRG3, SWI3

Background:

The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and contains a predicted leucine zipper motif typical of many transcription factors.

UniProt Protein Function:	SMARCC1: a core component of the BAF (SWI/SNF) complex. This family of complexes remodels chromatin structures, enabling transcription factors to gain access to DNA. They play important roles in cell proliferation and differentiation, in cellular antiviral activities and inhibition of tumor formation. The BAF complex is able to create a stable, altered form of chromatin that constrains fewer negative supercoils than normal. This change in supercoiling is due to the conversion of up to one-half of the nucleosomes on polynucleosomal arrays into asymmetric structures, termed altosomes, each composed of 2 histones octamers. Belongs to the neural progenitors- specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain BAF53A and PHF10, are exchanged for homologous alternative BAF53B and BAF45B or BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand- bound VDR-mediated transrepression of the CYP27B1 gene. Component of 6 multiprotein chromatin-remodeling complexes: Swi/Snf-A (BAF), Swi/Snf-B (PBAF), Brm, Brg1(I), WINAC and Brg1(II). Each of the five complexes contains a catalytic subunit (either SMARCA4 or SMARCA2), and at least SMARCE1, BAF53A or BAF53B, SMARCC2 and SMARCB1. Other subunits specific to each of the complexes may also be present. Component of the BAF (hSWI/SNF) complex, which includes at least actin (ACTB), ARID1A, ARID1B, SMARCA2, SMARCA4, BAF53A, BAF53B, SMARCE1 SMARCC1, SMARCC2, SMARCB1, and one or more of SMARCD1, SMARCD2, or SMARCD3. In muscle cells, the BAF complex also contains DPF3. May also interact with the SIN3A histone deacetylase transcription repressor complex in conjunction with SMARCA2 and SMARCA4. The minimal complex composed of SMARCC1 and SMARCA4 seems to be able to associate with cyclin such as CCNE1 or transcription factors such as KLF1 or GATA1. Expressed in brain, heart, muscle, placenta, lung, liver, muscle, kidney and pancreas. Belongs to the SMARCC family.
UniProt Protein Details:	Protein type:DNA-binding Chromosomal Location of Human Ortholog: 3p21.31 Cellular Component: nuclear chromatin; nucleoplasm; protein complex; SWI/SNF complex Molecular Function:nucleosomal DNA binding; protein binding; protein N-terminus binding Biological Process: ATP-dependent chromatin remodeling; chromatin remodeling; nucleosome disassembly; positive regulation of transcription, DNA-dependent
NCBI Summary:	The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and contains a predicted leucine zipper motif typical of many transcription factors. [provided by RefSeq, Jul 2008]
UniProt Code:	Q92922
NCBI GenInfo Identifier:	209572723
NCBI Gene ID:	6599
NCBI Accession:	Q92922.3
UniProt Secondary Accession:	Q92922,Q17RS0, Q6P172, Q8IWH2,
UniProt Related Accession:	Q92922
Molecular Weight:	122,867 Da
NCBI Full Name:	SWI/SNF complex subunit SMARCC1
NCBI Synonym Full Names:	SWI/SNF related, matrix associated, actin dependent regulator of chromatin subfamily c member 1
NCBI Official Symbol:	SMARCC1
NCBI Official Synonym Symbols:	Rsc8; SRG3; SWI3; BAF155; CRACC1
NCBI Protein Information:	SWI/SNF complex subunit SMARCC1
UniProt Protein Name:	SWI/SNF complex subunit SMARCC1
UniProt Synonym Protein Names:	BRG1-associated factor 155; BAF155; SWI/SNF complex 155 kDa subunit; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1
Protein Family:	SWI/SNF complex
UniProt Gene Name:	SMARCC1
UniProt Entry Name:	SMRC1_HUMAN

	Western blot analysis of extracts of 293T cells, using [KO Validated] SMARCC1 antibody (CAB6128) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded human kidney cancer using SMARCC1 antibody (CAB6128) at dilution of 1:200 (40x lens).
	Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug SMARCC1 antibody (CAB6128). Western blot was performed from the immunoprecipitate using SMARCC1 antibody (CAB6128) at a dilition of 1:1000.

Antibodies

Anti-SMARCC1/BAF155 Antibody (CAB4275)

SMARCC1 Antibody (PACO12333)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)