Anti-KRAS Antibody (CAB12704)

Antibody Name:	Anti-KRAS Antibody [KO Validated]
Antibody SKU:	CAB12704
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 30-188 of human KRAS (NP_004976.2).

Application:	WB IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SW620, C6, 293T, Mouse brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 30-188 of human KRAS (NP_004976.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	DEYD PTIE DSYR KQVV IDGE TCLL DILD TAGQ EEYS AMRD QYMR TGEG FLCV FAIN NTKS FEDI HHYR EQIK RVKD SEDV PMVL VGNK CDLP SRTV DTKQ AQDL ARSY GIPF IETS AKTR QGVD DAFY TLVR EIRK HKEK MSKD GKKK KKKS KTKC VIM
Gene ID:	3845
Uniprot:	P01116
Cellular Location:	Cell membrane, Cytoplasm, Cytoplasmic side, Lipid-anchor, cytosol
Calculated MW:	21kDa
Observed MW:	21KDa

Synonyms:

KRAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, KRAS2, NS, NS3, RALD, RASK2, c-Ki-ras2

Background:

This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region.

UniProt Protein Function:	KRas: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Interacts with PHLPP. Interacts (active GTP-bound form preferentially) with RGS14. Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase- activating protein (GAP). Belongs to the small GTPase superfamily. Ras family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Oncoprotein; G protein; G protein, monomeric; G protein, monomeric, Ras; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 12p12.1 Cellular Component: extrinsic to internal side of plasma membrane; focal adhesion; mitochondrion; membrane; cytoplasm; plasma membrane; cytosol; lipid raft Molecular Function:protein binding; GDP binding; GTP binding; GMP binding; protein complex binding; LRR domain binding Biological Process: regulation of long-term neuronal synaptic plasticity; axon guidance; nerve growth factor receptor signaling pathway; activation of MAPKK activity; response to glucocorticoid stimulus; activation of NF-kappaB transcription factor; positive regulation of MAP kinase activity; small GTPase mediated signal transduction; positive regulation of cell proliferation; visual learning; negative regulation of neuron apoptosis; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; cytokine and chemokine mediated signaling pathway; positive regulation of nitric-oxide synthase activity; MAPKKK cascade; social behavior; response to mineralocorticoid stimulus; regulation of synaptic transmission, GABAergic; positive regulation of Rac protein signal transduction; negative regulation of cell differentiation; Ras protein signal transduction; insulin receptor signaling pathway; innate immune response; striated muscle cell differentiation; positive regulation of protein amino acid phosphorylation; actin cytoskeleton organization and biogenesis; blood coagulation; vascular endothelial growth factor receptor signaling pathway; leukocyte migration Disease: Bladder Cancer; Gastric Cancer, Hereditary Diffuse; Pancreatic Cancer; Noonan Syndrome 3; Leukemia, Acute Myeloid; Lung Cancer; Breast Cancer; Schimmelpenning-feuerstein-mims Syndrome; Cardiofaciocutaneous Syndrome 2
NCBI Summary:	This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008]
UniProt Code:	P01116
NCBI GenInfo Identifier:	131875
NCBI Gene ID:	3845
NCBI Accession:	P01116.1
UniProt Related Accession:	P01116
Molecular Weight:
NCBI Full Name:	GTPase KRas
NCBI Synonym Full Names:	KRAS proto-oncogene, GTPase
NCBI Official Symbol:	KRAS
NCBI Official Synonym Symbols:	NS; NS3; CFC2; RALD; K-Ras; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; c-Ki-ras2
NCBI Protein Information:	GTPase KRas
UniProt Protein Name:	GTPase KRas
UniProt Synonym Protein Names:	K-Ras 2; Ki-Ras; c-K-ras; c-Ki-rasGTPase KRas, N-terminally processed
Protein Family:	GTPase
UniProt Gene Name:	KRAS
UniProt Entry Name:	RASK_HUMAN

	Western blot analysis of extracts from normal (control) and KRAS knockout (KO) 293T cells, using KRAS antibody (CAB12704) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min.
	Western blot analysis of extracts of various cell lines, using KRAS antibody (CAB12704) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunoprecipitation analysis of 600ug extracts of Mouse brain cells using 3ug KRAS antibody (CAB12704). Western blot was performed from the immunoprecipitate using KRAS antibody (CAB12704) at a dilition of 1:1000.

Antibodies	ELISA Kits
Anti-KRAS Antibody (CAB1190)	Human KRAS (GTPase KRas) ELISA Kit (HUFI03662)
Anti-Phospho-KRAS-S89 Antibody (CABP1168)
KRAS Antibody (PACO10157)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)