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[KO Validated] Lamin A/C Rabbit Polyclonal Antibody (CAB0249)

SKU:
CAB0249
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
  • KO Validated Antibodies 1 Anti-Lamin A/C Antibody CAB0249KO Validated
  • KO Validated Antibodies 1 Anti-Lamin A/C Antibody CAB0249KO Validated
  • KO Validated Antibodies 1 Anti-Lamin A/C Antibody CAB0249KO Validated
  • KO Validated Antibodies 1 Anti-Lamin A/C Antibody CAB0249KO Validated
  • KO Validated Antibodies 1 Anti-Lamin A/C Antibody CAB0249KO Validated
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:[KO Validated] Lamin A/C Rabbit Polyclonal Antibody
SKU:CAB0249
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 403-572 of human Lamin A/C (NP_733821.1).
Sequence:SSHS SQTQ GGGS VTKK RKLE STES RSSF SQHA RTSG RVAV EEVD EEGK FVRL RNKS NEDQ SMGN WQIK RQNG DDPL LTYR FPPK FTLK AGQV VTIW AAGA GATH SPPT DLVW KAQN TWGC GNSL RTAL INST GEEV AMRK LVRS VTVV EDDE DEDG DDLL HHHH GSHC SS
Tested Applications:WB IHC-P IF/ICC ChIP ELISA
Recommended Dilution:WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 ChIP,1:20 - 1:100
Synonyms:FPL; IDC; LFP; CDDC; EMD2; FPLD; HGPS; LDP1; LMN1; LMNC; MADA; PRO1; CDCD1; CMD1A; FPLD2; LMNL1; CMT2B1; LGMD1B; Lamin A/C
Positive Sample:HeLa,NIH/3T3,PC-12
Conjugate:Unconjugated
Cellular Localization:Nucleus, Nucleus envelope, Nucleus lamina, Nucleus speckle, nucleoplasm.
Calculated MW:74kDa
Observed MW:68kDa/72kDa

The protein encoded by this gene is part of the nuclear lamina, a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome.

Purification Method:Affinity purification
Gene ID:4000
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates, using Lamin A/C Rabbit pAb (CAB0249) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

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