The MEK (Methionine Enkephalin) ELISA Kit is specifically designed for the precise detection of Methionine Enkephalin levels in various biological samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it an ideal tool for a wide range of research applications.Methionine Enkephalin is a neuropeptide that plays a crucial role in pain modulation, neuroprotection, and immune regulation. Its levels have been implicated in various physiological and pathological conditions, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.Overall, the MEK ELISA Kit offers researchers a reliable and efficient way to measure Methionine Enkephalin levels, advancing our understanding of its roles in health and disease.
Product Name:
MEK (Methionine-Enkephalin) ELISA Kit
SKU:
UNES00015
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
75 pg/mL
Detection range:
125-8000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
