Description
Mouse Cytokine Array (22 targets) (SARB0078)
The Mouse Cytokine Array (SAR-B0078) is a comprehensive research tool designed for the simultaneous detection and quantification of 22 cytokine targets in mouse samples. This array enables researchers to analyze cytokine levels in a high-throughput and cost-effective manner, providing valuable insights into the immune response and inflammatory processes.Each array contains a panel of capture antibodies immobilized on a solid support, allowing for the specific binding of target cytokines present in the sample. The array can be used with a variety of sample types, including serum, plasma, cell culture supernatants, and tissue lysates, making it versatile for different experimental needs.
Whether studying inflammatory diseases, immune responses, or cancer biology, the Mouse Cytokine Array offers a powerful tool for researchers looking to profile cytokine expression patterns and identify potential therapeutic targets. With its high sensitivity and reproducibility, this array is a valuable asset for any laboratory conducting cytokine profiling studies in mouse models.
| Product Sku: | SARB0078 |
| Size: | 2, 4, or 8 |
| Species Detected: | Mouse |
| Number of Targets Detected: | 22 |
| Gene Symbols: | CCL12, CCL2, CCL5, CSF2, CSF3, IFNG, IL10, IL12A, IL13, IL17A|IL2, IL3, IL4, IL5, IL6, IL9, KITLG, THPO, TNF, TNFRSF1A, VEGFA |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
GCSF
| GM-CSF
| IL-2
| IL-3
| IL-4
|
IL-5
| IL-6
| IL-9
| IL-10
| IL-12 p40/p70
|
IL-12 p70
| IL-13
| IL-17A
| IFN-gamma
| MCP-1 (CCL2)
|
MCP-5
| RANTES (CCL5)
| SCF
| TNF RI (TNFRSF1A)
| TNF alpha
|
Thrombopoietin (TPO)
| VEGF-A
| |||
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Mouse Cytokine Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
