Description
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Mouse Cytokine Array (96 targets) (SARB0077)
The Mouse Cytokine Array (96 targets) (SARB0077) is a powerful tool for researchers studying cytokine expression in biological samples. This array allows for the simultaneous detection of 96 different cytokines in a single experiment, providing comprehensive information on the cytokine profile present in a sample.Each array is carefully designed to ensure high sensitivity and specificity, allowing for accurate and reliable results. With a wide range of cytokines covered, including key immune response regulators, growth factors, chemokines, and more, this array is ideal for a wide range of research applications in immunology, inflammation, cancer, and beyond.
Using this Mouse Cytokine Array, researchers can quickly and easily screen multiple cytokines in their samples, saving time and resources compared to traditional methods. This array is a valuable tool for gaining insights into the complex cytokine networks at play in various biological systems, ultimately leading to a deeper understanding of disease processes and potential therapeutic targets.
| Product Sku: | SARB0077 |
| Size: | 2, 4, or 8 |
| Species Detected: | Mouse |
| Number of Targets Detected: | 96 |
| Gene Symbols: | AXL, CCL1, CCL11, CCL12, CCL17, CCL19, CCL2, CCL20, CCL22, CCL24|CCL25, CCL27, CCL3, CCL5, CCL9, CD40, CSF1, CSF2, CSF2RB, CSF3, CX3CL1, CXCL1, CXCL10, CXCL11, CXCL12, CXCL13, CXCL15, CXCL16, CXCL2, CXCL5, CXCL9, DPP4, FASLG, FCGR2B, FGF2, FIGF, FLT1, FLT3LG, FLT4, ICAM1, IFNG, IGF1, IGF2, IGFBP2, IGFBP3, IGFBP5, IGFBP6, IL10, IL12A, IL13, IL15, IL17A, IL17RB, IL1A, IL1B, IL2, IL3, IL4, IL5, IL6, IL7, IL9, KDR, KITLG, LEP, LEPR, MET, MMP2, MMP3, MMP9, PF4, PPBP, RETN, SELE, SELL, SELP, SHH, SPP1, THPO, TIMP1, TIMP2, TNF, TNFRSF11B, TNFRSF18, TNFRSF19, TNFRSF8, TNFSF11, TNFSF8, TPO, TSLP, TYRO3, VCAM1, VEGFA, XCL1 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
Axl
| bFGF
| BLC (CXCL13)
| CD30 Ligand (TNFSF8)
| CD30 (TNFRSF8)
|
CD40 (TNFRSF5)
| CRG-2
| CTACK (CCL27)
| CXCL16
| CD26 (DPPIV)
|
Dtk
| Eotaxin-1 (CCL11)
| Eotaxin-2 (MPIF-2/CCL24)
| E-Selectin
| Fas Ligand (TNFSF6)
|
Fc gamma RIIB (CD32b)
| Flt-3 Ligand
| Fractalkine (CX3CL1)
| GCSF
| GITR (TNFRSF18)
|
GM-CSF
| HGFR
| ICAM-1 (CD54)
| IFN-gamma
| IGFBP-2
|
IGFBP-3
| IGFBP-5
| IGFBP-6
| IGF-1
| IGF-2
|
IL-1 beta (IL-1 F2)
| IL-10
| IL-12 p40/p70
| IL-12 p70
| IL-13
|
IL-15
| IL-17A
| IL-17 RB
| IL-1 alpha (IL-1 F1)
| IL-2
|
IL-3
| IL-3 R beta
| IL-4
| IL-5
| IL-6
|
IL-7
| IL-9
| I-TAC (CXCL11)
| KC (CXCL1)
| Leptin
|
Leptin R
| LIX
| L-Selectin (CD62L)
| Lungkine (CXCL15)
| Lymphotactin (XCL1)
|
MCP-1 (CCL2)
| MCP-5
| M-CSF
| MDC (CCL22)
| MIG (CXCL9)
|
MIP-1 alpha (CCL3)
| MIP-1 gamma
| MIP-2
| MIP-3 beta (CCL19)
| MIP-3 alpha (CCL20)
|
MMP-2
| MMP-3
| Osteopontin (SPP1)
| Osteoprotegerin (TNFRSF11B)
| Platelet Factor 4 (CXCL4)
|
Pro-MMP-9
| P-Selectin
| RANTES (CCL5)
| Resistin
| SCF
|
SDF-1 alpha (CXCL12 alpha)
| Sonic Hedgehog N-Terminal (Shh-N)
| TNF RI (TNFRSF1A)
| TNF RII (TNFRSF1B)
| TARC (CCL17)
|
I-309 (TCA-3/CCL1)
| TECK (CCL25)
| TCK-1 (CXCL7)
| TIMP-1
| TIMP-2
|
TNF alpha
| Thrombopoietin (TPO)
| TRANCE (TNFSF11)
| TROY (TNFRSF19)
| TSLP
|
VCAM-1 (CD106)
| VEGF-A
| VEGFR1
| VEGFR2
| VEGFR3
|
VEGF-D
| ||||
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Mouse Cytokine Antibody Array C1000 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
