The Mouse KYN (Kynurenine) ELISA Kit is specifically designed for the precise measurement of kynurenine levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and consistent results, making it an invaluable tool for various research purposes. Kynurenine is a key metabolite in the tryptophan pathway and has been linked to various immune and neurological disorders, including depression, schizophrenia, and autoimmune diseases.
By measuring kynurenine levels, researchers can gain insights into the pathophysiology of these conditions and potentially discover new therapeutic targets. This ELISA kit offers a reliable and convenient method for studying kynurenine metabolism and its role in health and disease. Its user-friendly format and quick protocol make it suitable for both experienced researchers and beginners in the field. Unlock the potential of your research with the Mouse KYN (Kynurenine) ELISA Kit.
Product Name:
Mouse KYN (Kynurenine) ELISA Kit
SKU:
MOFI01315
Reactivity:
Mouse
Assay Type:
Competitive ELISA, Coated with Antigen
Sensitivity:
4.688 pmol/mL
Range:
7.813-500 pmol/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C(Sealed), Don't cryopreserve.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 10)
93-105%
89-104%
93-101%
EDTA Plasma (n = 10)
83-100%
83-101%
84-100%
Heparin Plasma (n = 10)
90-98%
81-99%
80-99%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 10)
89-104
98
EDTA Plasma (n = 10)
90-104
97
Heparin Plasma (n = 10)
85-104
92
Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate TMB substrate for 30 minutes at room temperature. Prepare standards, samples (minimum 1:2 dilution), blanks, assign wells, and pre-wash the plate twice.
2
Sample & Biotin-Antibody Binding: Add 50 µL standard or sample followed by 50 µL biotin-labeled antibody to each well. Mix gently and incubate at 37°C for 45 minutes.
3
Washing: Wash the plate 5 times with wash buffer, allowing 1 minute soak time per wash.
4
Color Development: Add TMB substrate and incubate in the dark at 37°C for 10-20 minutes until color develops.
5
Stop Reaction: Add stop solution to terminate the reaction. The color changes from blue to yellow immediately.
6
Reading: Measure absorbance at 450 nm using a microplate reader.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant and store appropriately.
Plasma
Collect using EDTA anticoagulant, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Cell Culture Supernatant
Centrifuge at 1000 × g for 20 minutes at 4°C and collect clarified supernatant.
Cell Lysate
Lyse cells using recommended lysis buffer with protease inhibitors, centrifuge at 10,000 rpm for 10 minutes, and collect protein supernatant.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge at 5000 × g for 5 minutes, and collect supernatant.
Other Sample Types
Centrifuge samples at 1000 × g for 15 minutes at 2–8°C and collect supernatant. For additional guidance, please contact techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Biotin-labeled Antibody (Lyophilized)
1 vial
1 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
HRP-Streptavidin Conjugate (SABC, 100X)
60 ul
120 ul
2-8°C (Avoid direct light)
TMB Substrate
5 ml
10 ml
2-8°C (Avoid direct light)
Purified Water
200 ul
200 ul
2-8°C
Sample Dilution Buffer
10 ml
20 ml
2-8°C
Antibody Dilution Buffer
5 ml
10 ml
2-8°C
SABC Dilution Buffer
5 ml
10 ml
2-8°C
Stop Solution
5 ml
5 ml
2-8°C
Wash Buffer(25X)
15 ml
30 ml
2-8°C
Plate Sealer
3 pieces
5 pieces
-
Technical Manual
1 copy
1 copy
-
da Rocha et al.
Exhaustive exercise abolishes REV-ERB-α circadian rhythm and shifts the kynurenine pathway to a neurotoxic profile in mice
