The MTPAP Antibody (CAB17715) is a high-quality antibody developed for reliable detection and analysis of target proteins. This rabbit polyclonal antibody is highly specific and reactive with human samples, making it an excellent choice for Western blot applications. By binding to the MTPAP protein, this antibody enables the detection and analysis of MTPAP in various cell types, allowing for detailed research in the fields of mitochondrial biology and RNA metabolism.MTPAP is an essential enzyme involved in polyadenylation, a process crucial for the maturation and stability of mitochondrial mRNA.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
MTPAP Antibody
SKU:
CAB17715
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PAPD1, SPAX4, TENT6, MTPAP
Positive Sample:
U-87MG, U-251MG, Jurkat, Mouse spleen
Cellular Localization:
Mitochondrion, Nucleoplasm.
Calculated MW:
66kDa
Observed MW:
60kDa
The protein encoded by this gene is a member of the DNA polymerase type-B-like family. This enzyme synthesizes the 3' poly(A) tail of mitochondrial transcripts and plays a role in replication-dependent histone mRNA degradation.
Purification Method
Affinity purification
Gene ID
55149
RRID
AB_2770476
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using MTPAP Rabbit pAb (CAB17715) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
