Anti-MYOZ2 Antibody (CAB6468)

Antibody Name:	Anti-MYOZ2 Antibody
Antibody SKU:	CAB6468
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-264 of human MYOZ2 (NP_057683.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse heart, Mouse skeletal muscle, Rat skeletal muscle

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-264 of human MYOZ2 (NP_057683.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MLSH NTMM KQRK QQAT AIMK EVHG NDVD GMDL GKKV SIPR DIML EELS HLSN RGAR LFKM RQRR SDKY TFEN FQYQ SRAQ INHS IAMQ NGKV DGSN LEGG SQQA PLTP PNTP DPRS PPNP DNIA PGYS GPLK EIPP EKFN TTAV PKYY QSPW EQAI SNDP ELLE ALYP KLFK PEGK AELP DYRS FNRV ATPF GGFE KASR MVKF KVPD FELL LLTD PRFM SFVN PLSG RRSF NRTP KGWI SENI PIVI TTEP TDDT TVPE SEDL
Gene ID:	51778
Uniprot:	Q9NPC6
Cellular Location:	Cytoplasm, Z line, myofibril, sarcomere
Calculated MW:	29kDa
Observed MW:	35kDa

Synonyms:

MYOZ2, C4orf5, CMH16, CS-1

Background:

The protein encoded by this gene belongs to a family of sarcomeric proteins that bind to calcineurin, a phosphatase involved in calcium-dependent signal transduction in diverse cell types. These family members tether calcineurin to alpha-actinin at the z-line of the sarcomere of cardiac and skeletal muscle cells, and thus they are important for calcineurin signaling. Mutations in this gene cause cardiomyopathy familial hypertrophic type 16, a hereditary heart disorder.

UniProt Protein Function:	MYOZ2: Myozenins may serve as intracellular binding proteins involved in linking Z line proteins such as alpha-actinin, gamma- filamin, TCAP/telethonin, LDB3/ZASP and localizing calcineurin signaling to the sarcomere. Plays an important role in the modulation of calcineurin signaling. May play a role in myofibrillogenesis. Defects in MYOZ2 are the cause of familial hypertrophic cardiomyopathy type 16 (CMH16). CMH16 is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. Belongs to the myozenin family.
UniProt Protein Details:	Protein type:Contractile; Cytoskeletal Chromosomal Location of Human Ortholog: 4q26-q27 Cellular Component: sarcomere; Z disc; actin cytoskeleton Molecular Function:protein binding; telethonin binding; actin binding; protein phosphatase 2B binding Disease: Cardiomyopathy, Familial Hypertrophic, 16
NCBI Summary:	The protein encoded by this gene belongs to a family of sarcomeric proteins that bind to calcineurin, a phosphatase involved in calcium-dependent signal transduction in diverse cell types. These family members tether calcineurin to alpha-actinin at the z-line of the sarcomere of cardiac and skeletal muscle cells, and thus they are important for calcineurin signaling. Mutations in this gene cause cardiomyopathy familial hypertrophic type 16, a hereditary heart disorder. [provided by RefSeq, Aug 2011]
UniProt Code:	Q9NPC6
NCBI GenInfo Identifier:	74761638
NCBI Gene ID:	51778
NCBI Accession:	Q9NPC6.1
UniProt Secondary Accession:	Q9NPC6,O43415, Q9HB92,
UniProt Related Accession:	Q9NPC6
Molecular Weight:	264
NCBI Full Name:	Myozenin-2
NCBI Synonym Full Names:	myozenin 2
NCBI Official Symbol:	MYOZ2
NCBI Official Synonym Symbols:	CS-1; CMH16; C4orf5
NCBI Protein Information:	myozenin-2; MYOZ2; FATZ-related protein 2; muscle-specific protein; calcineurin-binding protein calsarcin-1
UniProt Protein Name:	Myozenin-2
UniProt Synonym Protein Names:	Calsarcin-1; FATZ-related protein 2
Protein Family:	Myozenin
UniProt Gene Name:	MYOZ2
UniProt Entry Name:	MYOZ2_HUMAN

	Western blot analysis of extracts of various cell lines, using MYOZ2 antibody (CAB6468) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunofluorescence analysis of U2OS cells using MYOZ2 antibody (CAB6468). Blue: DAPI for nuclear staining.

Antibodies

MYOZ2 Antibody (PACO07292)

MYOZ2 Antibody (PACO10713)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)