Anti-NDUFS8 Antibody (CAB13034)

Antibody Name:	Anti-NDUFS8 Antibody
Antibody SKU:	CAB13034
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 35-210 of human NDUFS8 (NP_002487.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse brain, Mouse heart, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 35-210 of human NDUFS8 (NP_002487.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	TYKY VNMQ DPEM DMKS VTDR AART LLWT ELFR GLGM TLSY LFRE PATI NYPF EKGP LSPR FRGE HALR RYPS GEER CIAC KLCE AICP AQAI TIEA EPRA DGSR RTTR YDID MTKC IYCG FCQE ACPV DAIV EGPN FEFS TETH EELL YNKE KLLN NGDK WEAE IAAN IQAD YLYR
Gene ID:	4728
Uniprot:	O00217
Cellular Location:	Mitochondrion
Calculated MW:	23kDa
Observed MW:	23kDa

Synonyms:

NDUFS8, CI-23k, CI23KD, TYKY

Background:

This gene encodes a subunit of mitochondrial NADH:ubiquinone oxidoreductase, or Complex I, a multimeric enzyme of the respiratory chain responsible for NADH oxidation, ubiquinone reduction, and the ejection of protons from mitochondria. The encoded protein is involved in the binding of two of the six to eight iron-sulfur clusters of Complex I and, as such, is required in the electron transfer process. Mutations in this gene have been associated with Leigh syndrome.

UniProt Protein Function:	TYKY: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. May donate electrons to ubiquinone. Defects in NDUFS8 are a cause of Leigh syndrome (LS). LS is a severe neurological disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions. Belongs to the complex I 23 kDa subunit family.
UniProt Protein Details:	Protein type:EC 1.6.99.3; EC 1.6.5.3; Mitochondrial; Energy Metabolism - oxidative phosphorylation; Oxidoreductase Chromosomal Location of Human Ortholog: 11q13 Cellular Component: mitochondrial matrix; mitochondrial respiratory chain complex I; mitochondrion Molecular Function:NADH dehydrogenase (ubiquinone) activity; NADH dehydrogenase activity Biological Process: mitochondrial electron transport, NADH to ubiquinone; mitochondrial respiratory chain complex I assembly; response to oxidative stress Disease: Leigh Syndrome
NCBI Summary:	This gene encodes a subunit of mitochondrial NADH:ubiquinone oxidoreductase, or Complex I, a multimeric enzyme of the respiratory chain responsible for NADH oxidation, ubiquinone reduction, and the ejection of protons from mitochondria. The encoded protein is involved in the binding of two of the six to eight iron-sulfur clusters of Complex I and, as such, is required in the electron transfer process. Mutations in this gene have been associated with Leigh syndrome. [provided by RefSeq, Mar 2010]
UniProt Code:	O00217
NCBI GenInfo Identifier:	2499325
NCBI Gene ID:	4728
NCBI Accession:	O00217.1
UniProt Secondary Accession:	O00217,Q0VDA8, B2RB86,
UniProt Related Accession:	O00217
Molecular Weight:	23,705 Da
NCBI Full Name:	NADH dehydrogenase
NCBI Synonym Full Names:	NADH:ubiquinone oxidoreductase core subunit S8
NCBI Official Symbol:	NDUFS8
NCBI Official Synonym Symbols:	TYKY; CI-23k; CI23KD
NCBI Protein Information:	NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial
UniProt Protein Name:	NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial
UniProt Synonym Protein Names:	Complex I-23kD; CI-23kD; NADH-ubiquinone oxidoreductase 23 kDa subunit; TYKY subunit
UniProt Gene Name:	NDUFS8
UniProt Entry Name:	NDUS8_HUMAN

	Western blot analysis of extracts of various cell lines, using NDUFS8 antibody (CAB13034) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of L929 cells using NDUFS8 Polyclonal Antibody (CAB13034) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ELISA Kits

Human NDUFS8 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial ELISA Kit

Human NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial (NDUFS8) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)