Anti-OAT Antibody (CAB6235)

Antibody Name:	Anti-OAT Antibody
Antibody SKU:	CAB6235
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 160-439 of human OAT (NP_000265.1).

Application:	WB IHC IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	SW480, MCF7, 293T, A-549, HepG2, Mouse liver, Mouse lung, Mouse kidney, Rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 160-439 of human OAT (NP_000265.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	VKGI QKYK AKIV FAAG NFWG RTLS AISS STDP TSYD GFGP FMPG FDII PYND LPAL ERAL QDPN VAAF MVEP IQGE AGVV VPDP GYLM GVRE LCTR HQVL FIAD EIQT GLAR TGRW LAVD YENV RPDI VLLG KALS GGLY PVSA VLCD DDIM LTIK PGEH GSTY GGNP LGCR VAIA ALEV LEEE NLAE NADK LGII LRNE LMKL PSDV VTAV RGKG LLNA IVIK ETKD WDAW KVCL RLRD NGLL AKPT HGDI IRFA PPLV IKED ELRE SIEI INKT ILSF
Gene ID:	4942
Uniprot:	P04181
Cellular Location:	Mitochondrion matrix
Calculated MW:	32kDa/48kDa
Observed MW:	47kDa

Synonyms:

OAT, GACR, HOGA, OATASE, OKT

Background:

This gene encodes the mitochondrial enzyme ornithine aminotransferase, which is a key enzyme in the pathway that converts arginine and ornithine into the major excitatory and inhibitory neurotransmitters glutamate and GABA. Mutations that result in a deficiency of this enzyme cause the autosomal recessive eye disease Gyrate Atrophy. Alternatively spliced transcript variants encoding different isoforms have been described. Related pseudogenes have been defined on the X chromosome.

UniProt Protein Function:	OAT: Defects in OAT are the cause of hyperornithinemia with gyrate atrophy of choroid and retina (HOGA). HOGA is a slowly progressive blinding autosomal recessive disorder. Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Amino Acid Metabolism - arginine and proline; EC 2.6.1.13; Transferase; Mitochondrial Chromosomal Location of Human Ortholog: 10q26 Cellular Component: mitochondrial matrix; mitochondrion Molecular Function:identical protein binding; ornithine-oxo-acid transaminase activity; pyridoxal phosphate binding Biological Process: amino acid biosynthetic process; arginine catabolic process to glutamate; arginine catabolic process to proline via ornithine; visual perception Disease: Gyrate Atrophy Of Choroid And Retina
NCBI Summary:	This gene encodes the mitochondrial enzyme ornithine aminotransferase, which is a key enzyme in the pathway that converts arginine and ornithine into the major excitatory and inhibitory neurotransmitters glutamate and GABA. Mutations that result in a deficiency of this enzyme cause the autosomal recessive eye disease Gyrate Atrophy. Alternatively spliced transcript variants encoding different isoforms have been described. Related pseudogenes have been defined on the X chromosome. [provided by RefSeq, Jan 2010]
UniProt Code:	P04181
NCBI GenInfo Identifier:	129018
NCBI Gene ID:	4942
NCBI Accession:	P04181.1
UniProt Secondary Accession:	P04181,Q16068, Q16069, Q68CS0, Q6IAV9, Q9UD03, D3DRF0
UniProt Related Accession:	P04181
Molecular Weight:	32,853 Da
NCBI Full Name:	Ornithine aminotransferase, mitochondrial
NCBI Synonym Full Names:	ornithine aminotransferase
NCBI Official Symbol:	OAT
NCBI Official Synonym Symbols:	OKT; GACR; HOGA; OATASE
NCBI Protein Information:	ornithine aminotransferase, mitochondrial
UniProt Protein Name:	Ornithine aminotransferase, mitochondrial
UniProt Synonym Protein Names:	Ornithine delta-aminotransferase; Ornithine--oxo-acid aminotransferase
UniProt Gene Name:	OAT
UniProt Entry Name:	OAT_HUMAN

	Western blot analysis of extracts of various cell lines, using OAT antibody (CAB6235) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded rat kidney using OAT Rabbit pAb (CAB6235) at dilution of 1:150 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophageal cancer using OAT Rabbit pAb (CAB6235) at dilution of 1:150 (40x lens).
	Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug OAT antibody (CAB6235). Western blot was performed from the immunoprecipitate using OAT antibody (CAB6235) at a dilition of 1:1000.

Antibodies	ELISA Kits
OAT Antibody (PACO04477)	Human ornithine aminotransferase / OAT ELISA Kit
	Human Ornithine aminotransferase, mitochondrial (OAT) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)