OPA1 Polyclonal Antibody (CAB9833)

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SKU:
CAB9833
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Description

Technical Manual

OPA1 Rabbit Polyclonal Antibody

Product Name:OPA1 Rabbit Polyclonal Antibody
Product SKU:CAB9833
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

This gene product is a nuclear-encoded mitochondrial protein with similarity to dynamin-related GTPases. It is a component of the mitochondrial network. Mutations in this gene have been associated with optic atrophy type 1, which is a dominantly inherited optic neuropathy resulting in progressive loss of visual acuity, leading in many cases to legal blindness. Multiple transcript variants encoding different isoforms have been found for this gene.

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 661-960 of human OPA1 (NP_056375.2).
Sequence:

TDKQLPNKAVEVAWETLQEEFSRFMTEPKGKEHDDIFDKLKEAVKEESIKRHKWNDFAEDSLRVIQHNALEDRSISDKQQWDAAIYFMEEALQARLKDTENAIENMVGPDWKKRWLYWKNRTQEQCVHNETKNELEKMLKCNEEHPAYLASDEITTVRKNLESRGVEVDPSLIKDTWHQVYRRHFLKTALNHCNLCRRGFYYYQRHFVDSELECNDVVLFWRIQRMLAITANTLRQQLTNTEVRRLEKNVKEVLEDFAEDGEKKIKLLTGKRVQLAEDLKKVREIQEKLDAFIEALHQEK

Gene ID:4976
Swiss Prot:O60313
Synonyms:OPA1; BERHS; MGM1; MTDPS14; NPG; NTG; largeG
Calculated MW:111kDa/115kDa
Observed MW:80-100KDa
Reactivity:Human, Mouse, Rat
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting Immunofluorescence 
Positive Samples:HeLa, HepG2, U-251MG, Mouse liver, Mouse brain, Mouse heart, Rat liver, Rat brain
Cellular Location:Mitochondrion inner membrane, Mitochondrion intermembrane space, Single-pass membrane protein

Product Images

OPA1 Polyclonal Antibody (CAB9833)

Western blot analysis of extracts of various cell lines, using OPA1 Rabbit pAb (CAB9833) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.

Uniprot Information

UniProt Protein Function:OPA1: a dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space. Defects in OPA1 are the cause of optic atrophy 1 and deafness. Eight splice-variant isoforms have been described.
UniProt Protein Details:

Protein type:Apoptosis; EC 3.6.5.5; Hydrolase; Membrane protein, integral; Mitochondrial

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: cytosol; dendrite; membrane; mitochondrial crista; mitochondrial inner membrane; mitochondrial intermembrane space; mitochondrial outer membrane; mitochondrion; nucleoplasm

Molecular Function:GTPase activity; microtubule binding; protein binding

Biological Process: axon transport of mitochondrion; inner mitochondrial membrane organization and biogenesis; mitochondrial fission; mitochondrial fusion; mitochondrial genome maintenance; mitochondrion organization and biogenesis; regulation of apoptosis; visual perception

Disease: Behr Syndrome; Glaucoma, Normal Tension, Susceptibility To; Mitochondrial Dna Depletion Syndrome 14 (cardioencephalomyopathic Type); Optic Atrophy 1; Optic Atrophy With Or Without Deafness, Ophthalmoplegia, Myopathy, Ataxia, And Neuropathy

NCBI Summary:This gene product is a nuclear-encoded mitochondrial protein with similarity to dynamin-related GTPases. It is a component of the mitochondrial network. Mutations in this gene have been associated with optic atrophy type 1, which is a dominantly inherited optic neuropathy resulting in progressive loss of visual acuity, leading in many cases to legal blindness. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
UniProt Code:O60313
NCBI GenInfo Identifier:215274226
NCBI Gene ID:4976
NCBI Accession:O60313.3
UniProt Secondary Accession:O60313,D3DNW4,
UniProt Related Accession:O60313
Molecular Weight:112kDa
NCBI Full Name:Dynamin-like 120 kDa protein, mitochondrial
NCBI Synonym Full Names:OPA1, mitochondrial dynamin like GTPase
NCBI Official Symbol:OPA1 
NCBI Official Synonym Symbols:NPG; NTG; MGM1; BERHS; largeG; MTDPS14 
NCBI Protein Information:dynamin-like 120 kDa protein, mitochondrial
UniProt Protein Name:Dynamin-like 120 kDa protein, mitochondrial
UniProt Synonym Protein Names:Optic atrophy protein 1
Protein Family:Dynamin-like 120 kDa protein
UniProt Gene Name:OPA1 
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Additional Information

Product type:
Antibody
Application:
WB
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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