The P2RY14 Antibody (CAB8178) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly reactive with human samples and is validated for use in various applications, including Western blot and immunohistochemistry.The P2Y purinoceptor 14 protein plays a crucial role in mediating cellular responses to extracellular nucleotides, including ATP and ADP, which are important signaling molecules in processes such as inflammation, neurotransmission, and thrombosis. By targeting this receptor, researchers can gain insights into the regulation of these physiological processes and explore its implications in diseases such as cardiovascular disorders, neurodegenerative diseases, and immune disorders.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
P2RY14 Antibody
SKU:
CAB8178
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
P2Y14, BPR105, GPR105, P2RY14
Positive Sample:
K-562
Cellular Localization:
Cell Membrane, Multi-Pass Membrane Protein.
Calculated MW:
39kDa
Observed MW:
38kDa
The product of this gene belongs to the family of G-protein coupled receptors, which contains several receptor subtypes with different pharmacological selectivity for various adenosine and uridine nucleotides. This receptor is a P2Y purinergic receptor for UDP-glucose and other UDP-sugars coupled to G-proteins. It has been implicated in extending the known immune system functions of P2Y receptors by participating in the regulation of the stem cell compartment, and it may also play a role in neuroimmune function. Two transcript variants encoding the same protein have been identified for this gene.
Purification Method
Affinity purification
Gene ID
9934
RRID
AB_2770762
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from K-562 cells, using P2RY14 Rabbit pAb (CAB8178) at 1:1500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Rat ovary using P2RY14 Rabbit pAb (CAB8178) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
