p38 MAPK / SAPK-JNK Stress Antibody Sampler Pack

p38 MAPK / SAPK-JNK Stress Antibody Sampler Pack

The p38 MAPK / SAPK-JNK Stress Antibody Sampler Pack from Assay Genie brings together 8 rigorously validated antibodies spanning the key nodes of this pathway, supplied in a convenient 20µL trial size. It lets researchers screen an entire signalling axis in a single, cost-effective pack — validated for applications including ELISA, IF/ICC, IHC-P, IP, WB — before committing to full-size vials. Each antibody is also available individually in a full 100µL size.

The stress-activated protein kinase branches of the MAPK family, comprising p38 MAPK and the c-Jun N-terminal kinases, respond to environmental and inflammatory stresses such as ultraviolet radiation, osmotic shock, oxidative damage, and inflammatory cytokines, coordinating decisions between adaptation, cell-cycle arrest, and apoptosis. These cascades are organized as three-tiered kinase relays. Upstream MAP3Ks activate the MAP2Ks MKK3 and MKK6, whose activation is detectable as Phospho-MKK3/6, and these kinases in turn phosphorylate p38 MAPK to its active Phospho-p38 form. In parallel, MKK4 and MKK7 activate SAPK/JNK, generating Phospho-SAPK/JNK. The activated terminal kinases drive transcriptional reprogramming: JNK phosphorylates c-Jun to its transcriptionally active Phospho-c-Jun state, enhancing AP-1-dependent gene expression, while both p38 and JNK phosphorylate the transcription factor ATF2, which heterodimerizes with c-Jun to regulate stress-responsive promoters. The biological output of these pathways is highly context dependent, promoting either survival and inflammation or programmed cell death depending on signal duration and cellular setting. In cancer this duality is striking: sustained JNK and p38 activity can enforce tumor suppression through senescence and apoptosis, yet the same kinases can support tumor cell survival, invasion, inflammation-driven carcinogenesis, and therapy resistance in established malignancies. Because these pathways integrate chemotherapeutic and radiation-induced stress, their activation status influences treatment response. Profiling total and phosphorylated p38, SAPK/JNK, c-Jun, the substrate ATF2, and the upstream Phospho-MKK3/6 node together resolves the magnitude and direction of stress signaling in a given experimental or tumor context. This pack brings together validated antibodies against p38 MAPK, Phospho-p38, SAPK/JNK, Phospho-SAPK/JNK, c-Jun, Phospho-c-Jun, ATF2, and Phospho-MKK3/6 for studying the p38 MAPK and SAPK/JNK stress signaling pathways.