The PDLIM5 Polyclonal Antibody (CAB22013) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a member of a family of proteins that possess a 100-amino acid PDZ domain at the N terminus and one to three LIM domains at the C-terminus. This family member functions as a scaffold protein that tethers protein kinases to the Z-disk in striated muscles. It is thought to function in cardiomyocyte expansion and in restraining postsynaptic growth of excitatory synapses. Alternative splicing of this gene results in multiple transcript variants. RRID Gene ID 10611 Swiss Prot Synonym L9; ENH; LIM; ENH1; PDLIM5
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
PDLIM5 Polyclonal Antibody
SKU:
CAB22013
Size:
100μL, 20μL
Reactivity:
Human
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
L9, ENH, LIM, ENH1, PDLIM5
Positive Sample:
HeLa
Cellular Localization:
Actin Cytoskeleton, Cell Projection, Cytosol, Postsynaptic Density, Presynapse, Z Disc.
Calculated MW:
64kDa
Observed MW:
64kDa
This gene encodes a member of a family of proteins that possess a 100-amino acid PDZ domain at the N terminus and one to three LIM domains at the C-terminus. This family member functions as a scaffold protein that tethers protein kinases to the Z-disk in striated muscles. It is thought to function in cardiomyocyte expansion and in restraining postsynaptic growth of excitatory synapses. Alternative splicing of this gene results in multiple transcript variants. RRID Gene ID 10611 Swiss Prot Synonym L9; ENH; LIM; ENH1; PDLIM5
Purification Method:
Affinity purification
Gene ID:
10611
RRID:
-
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HeLa cells, using PDLIM5 Rabbit pAb (CAB22013) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
