Anti-PHLDA2 Antibody (CAB6244)

Antibody Name:	Anti-PHLDA2 Antibody
Antibody SKU:	CAB6244
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-152 of human PHLDA2 (NP_003302.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:20 - 1:100
Reactivity:	Human
Positive Samples:	SW620, SKOV3, HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-152 of human PHLDA2 (NP_003302.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MKSP DEVL REGE LEKR SDSL FQLW KKKR GVLT SDRL SLFP ASPR ARPK ELRF HSIL KVDC VERT GKYV YFTI VTTD HKEI DFRC AGES CWNA AIAL ALID FQNR RALQ DFRS RQER TAPA APAE DAVA AAAA APSE PSEP SRPS PQPK PRTP
Gene ID:	7262
Uniprot:	Q53GA4
Cellular Location:	Cytoplasm, Membrane, Peripheral membrane protein
Calculated MW:	17kDa
Observed MW:	20kDa

Synonyms:

PHLDA2, BRW1C, BWR1C, HLDA2, IPL, TSSC3

Background:

This gene is located in a cluster of imprinted genes on chromosome 11p15.5, which is considered to be an important tumor suppressor gene region. Alterations in this region may be associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. This gene has been shown to be imprinted, with preferential expression from the maternal allele in placenta and liver.

UniProt Protein Function:	PHLDA2: a protein may play a role in regulating placenta growth. Contains one PH domain. Expressed in placenta and adult prostate gland. Expressed at low levels in adult liver and lung, and fetal liver. Expressed in adult brain and neuroblastoma, medullablastoma and glioblastoma cell lines.
UniProt Protein Details:	Protein type:Unknown function Chromosomal Location of Human Ortholog: 11p15.4 Biological Process: apoptosis
NCBI Summary:	This gene is located in a cluster of imprinted genes on chromosome 11p15.5, which is considered to be an important tumor suppressor gene region. Alterations in this region may be associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. This gene has been shown to be imprinted, with preferential expression from the maternal allele in placenta and liver. [provided by RefSeq, Oct 2010]
UniProt Code:	Q53GA4
NCBI GenInfo Identifier:	84029394
NCBI Gene ID:	7262
NCBI Accession:	Q53GA4.2
UniProt Secondary Accession:	Q53GA4,O00496,
UniProt Related Accession:	Q53GA4
Molecular Weight:	17,092 Da
NCBI Full Name:	Pleckstrin homology-like domain family A member 2
NCBI Synonym Full Names:	pleckstrin homology like domain family A member 2
NCBI Official Symbol:	PHLDA2
NCBI Official Synonym Symbols:	IPL; BRW1C; BWR1C; HLDA2; TSSC3
NCBI Protein Information:	pleckstrin homology-like domain family A member 2
UniProt Protein Name:	Pleckstrin homology-like domain family A member 2
UniProt Synonym Protein Names:	Beckwith-Wiedemann syndrome chromosomal region 1 candidate gene C protein; Imprinted in placenta and liver protein; Tumor-suppressing STF cDNA 3 protein; Tumor-suppressing subchromosomal transferable fragment candidate gene 3 protein; p17-Beckwith-Wiedemann region 1 C; p17-BWR1C
Protein Family:	Pleckstrin homology-like domain family
UniProt Gene Name:	PHLDA2
UniProt Entry Name:	PHLA2_HUMAN

	Western blot analysis of extracts of various cell lines, using PHLDA2 antibody (CAB6244) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunofluorescence analysis of HeLa cells using PHLDA2 antibody (CAB6244). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-PHLDA2 Antibody (CAB13924)	Human Pleckstrin homology-like domain family A member 2 (PHLDA2) ELISA Kit
PHLDA2 Antibody (PACO05260)
PHLDA2 Antibody (PACO11220)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)