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Phospho-AKT1S1/PRAS40-T246 Rabbit Polyclonal Antibody (CABP0793)

SKU:
CABP0793
Product Type:
Antibody
Applications:
WB
IHC
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
€419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-AKT1S1/PRAS40-T246 Rabbit Polyclonal Antibody
SKU:CABP0793
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:A synthetic phosphorylated peptide around T246 of human AKT1S1/PRAS40 (NP_001092102.1).
Sequence:LNTS D
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:Lobe; PRAS40; Phospho-AKT1S1/PRAS40-T246
Positive Sample:HeLa
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, cytosol.
Calculated MW:27kDa
Observed MW:40kDa

AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).

Purification Method:Affinity purification
Gene ID:84335
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of HeLa, using Phospho-AKT1S1/PRAS40-T246 Rabbit pAb (CABP0793) at 1:400 dilution.HeLa cells were treated by Insulin (100 nM) at 37℃ for 5 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 45s.

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