The Phospho-GAB1-Y307 Polyclonal Antibody (CABP0961) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is a member of the IRS1-like multisubstrate docking protein family. It is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Two transcript variants encoding different isoforms have been found for this gene. RRID Gene ID 2549 Swiss Prot Synonym DFNB26; Phospho-GAB1-Y307
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
Phospho-GAB1-Y307 Polyclonal Antibody
SKU:
CABP0961
Size:
100μL, 20μL
Reactivity:
Mouse
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
DFNB26, Phospho-GAB1-Y307
Positive Sample:
NIH/3T3 treated with IGF-1
Cellular Localization:
Cell-Cell Junction, Cytoplasm, Cytosol.
Calculated MW:
77kDa
Observed MW:
115kDa
The protein encoded by this gene is a member of the IRS1-like multisubstrate docking protein family. It is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Two transcript variants encoding different isoforms have been found for this gene. RRID Gene ID 2549 Swiss Prot Synonym DFNB26; Phospho-GAB1-Y307
Purification Method:
Affinity purification
Gene ID:
2549
RRID:
-
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells using Phospho-GAB1-Y307 Rabbit pAb (CABP0961) at 1:1000 dilution. NIH/3T3 cells were treated with IGF-1 (50 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
