Western blot analysis of NIH/3T3, using Phospho-NF-κB2-S866 antibody (CABP1367) at 1:500 dilution. NIH/3T3 cells were treated by TNF-α (20 ng/ml) at 37℃ for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The Phospho-NF-kappaB2-S866 Monoclonal Antibody (CABP1367) is a high-quality antibody developed for reliable detection and analysis of target proteins. NF-κB2 is a transcription factor that plays a critical role in immune and inflammatory responses by regulating the expression of various genes involved in these processes. This antibody, produced using monoclonal technology, is optimized for use in Western blotting applications and is highly reactive with human samples. It specifically binds to the phosphorylated form of NF-κB2 at serine 866, allowing for the detection and analysis of this key signaling event in various cell types.The phosphorylation of NF-κB2 at serine 866 is known to be involved in the regulation of NF-κB activation and downstream gene expression, making it an important target for research in immunology and cancer biology.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
Phospho-NF-kappaB2-S866 Monoclonal Antibody
SKU:
CABP1367
Size:
20μL, 100μL
Reactivity:
Mouse
Clone Number:
ARC56344
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
EDSA Y
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:100 - 1:500
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFkB). The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The protein encoded by this gene can function as both a transcriptional activator or repressor depending on its dimerization partner. The p100 full-length protein is co-translationally processed into a p52 active form. Chromosomal rearrangements and translocations of this locus have been observed in B cell lymphomas, some of which may result in the formation of fusion proteins. There is a pseudogene for this gene on chromosome 18. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
4791
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-NF-κB2-S866 Rabbit mAb (CABP1367) at 1:500 dilution. NIH/3T3 cells were treated with TNF-α (20 ng/ml) at 37℃ for 30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
