Western blot analysis of 293T lysates, using Phospho-eNOS-S1177 antibody (CABP1404) at 1:7000 dilution. 293T cells were treated by IGF-1 (50 ng/ml) at 37℃ for 5 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The Phospho-eNOS-S1177 Monoclonal Antibody (CABP1404) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced using monoclonal technology, specifically recognizes the phosphorylated form of eNOS at serine 1177, a key regulatory site for eNOS activation. eNOS is an important enzyme involved in the production of nitric oxide, a signaling molecule that plays a critical role in regulating blood flow, vascular tone, and cardiovascular health. Phosphorylation of eNOS at S1177 is known to enhance its activity, leading to increased nitric oxide production and vasodilation.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-eNOS-S1177 Monoclonal Antibody
SKU:
CABP1404
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC58017
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
TQSF S
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:2000 - 1:8000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
4846
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HUVEC cells using Phospho-eNOS-S1177 Rabbit mAb (CABP1404) at 1:6000 dilution incubated overnight at 4℃. HUVEC cells were treated with Pervanadate (100 μM) at 37℃ for 10 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60 s.
