The Phospho-PP2A Catalytic alpha-Y307 Monoclonal Antibody (CABP1043) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. RRID AB_2863919 Gene ID 5515 Swiss Prot Synonym RP-C; PP2Ac; PP2CA; NEDLBA; PP2Calpha; Phospho-PP2A Catalytic α-Y307
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Chromosome, Cytoplasm, Nucleus, Centromere, Cytoskeleton, Spindle Pole.
Calculated MW:
36kDa
Observed MW:
35kDa
This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. RRID AB_2863919 Gene ID 5515 Swiss Prot Synonym RP-C; PP2Ac; PP2CA; NEDLBA; PP2Calpha; Phospho-PP2A Catalytic α-Y307
Purification Method:
Affinity purification
Gene ID:
5515
RRID:
AB_2863919
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-PP2A Catalytic α-Y307 Rabbit mAb (CABP1043) at 1:1000 dilution. NIH/3T3 cells were treated with Forskolin (30 uM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
