The Phospho-PP2A Catalytic alpha-Y307 Monoclonal Antibody (CABP1043) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to the phosphorylated form of PP2A alpha at tyrosine 307 and is suitable for use in Western blot applications.PP2A alpha is a well-known serine/threonine phosphatase that plays a critical role in cell signaling pathways by dephosphorylating key protein substrates. Phosphorylation of PP2A alpha at tyrosine 307 has been linked to its activation and regulation of downstream signaling cascades.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Chromosome, Cytoplasm, Nucleus, Centromere, Cytoskeleton, Spindle Pole.
Calculated MW:
36kDa
Observed MW:
35kDa
This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit.
Purification Method
Affinity purification
Gene ID
5515
RRID
AB_2863919
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-PP2A Catalytic α-Y307 Rabbit mAb (CABP1043) at 1:1000 dilution. NIH/3T3 cells were treated with Forskolin (30 uM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
