Anti-LKB1 Antibody (CAB2122)

Antibody Name:	Anti-LKB1 Antibody [KO Validated]
Antibody SKU:	CAB2122
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 134-433 of human LKB1 (NP_000446.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, SKOV3, Mouse heart, 293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 134-433 of human LKB1 (NP_000446.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	CGMQ EMLD SVPE KRFP VCQA HGYF CQLI DGLE YLHS QGIV HKDI KPGN LLLT TGGT LKIS DLGV AEAL HPFA ADDT CRTS QGSP AFQP PEIA NGLD TFSG FKVD IWSA GVTL YNIT TGLY PFEG DNIY KLFE NIGK GSYA IPGD CGPP LSDL LKGM LEYE PAKR FSIR QIRQ HSWF RKKH PPAE APVP IPPS PDTK DRWR SMTV VPYL EDLH GADE DEDL FDIE DDII YTQD FTVP GQVP EEEA SHNG QRRG LPKA VCMN GTEA AQLS TKSR AEGR APNP ARKA CSAS SKIR RLSA CKQQ
Gene ID:	6794
Uniprot:	Q15831
Cellular Location:	Cytoplasm, Membrane, Mitochondrion, Nucleus
Calculated MW:	45kDa/48kDa
Observed MW:	54kDa

Synonyms:

LKB1, PJS, hLKB1, STK11

Background:

This gene, which encodes a member of the serine/threonine kinase family, regulates cell polarity and functions as a tumor suppressor. Mutations in this gene have been associated with Peutz-Jeghers syndrome, an autosomal dominant disorder characterized by the growth of polyps in the gastrointestinal tract, pigmented macules on the skin and mouth, and other neoplasms. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.

UniProt Protein Function:	LKB1: a S/T protein kinase of the CAMKL family. A tumor suppressor that helps control cell structure, polarity, apoptosis and energy homeostasis. Phosphorylates AGS3 (activator of G-protein signaling 3) GPR domains, regulating the interaction of GPR-containing proteins with G-proteins. A cytosolic protein complex comprised of LKB1, the pseudokinase STRAD, and the MO25 scaffold protein. Activates AMPK and several related protein kinases. AMPK plays a predominant role as the master regulator of cellular energy homeostasis, controlling downstream effectors that regulate cell growth and apoptosis in response to cellular ATP concentrations. The Lkb1/Strad/Mo25 complex can polarize single epithelial cells, leading to the formation of a brush border containing microvilli on the apical surface. Mutation in the corresponding gene causes Peutz-Jeghers syndrome (PJS), an autosomal dominant disorder characterized by benign GI tract polyps and dark skin lesions of the mouth, hands and feet. A variety of other LKB1 gene mutations have been associated with the formation of sporadic cancers in several tissues.
UniProt Protein Details:	Protein type:Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor); Tumor suppressor; Autophagy; EC 2.7.11.1; Kinase, protein; CAMK group; CAMKL family; LKB subfamily Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: cytoplasm; cytosol; membrane; mitochondrion; nucleus Molecular Function:ATP binding; LRR domain binding; magnesium ion binding; p53 binding; protein binding; protein kinase activator activity; protein serine/threonine kinase activity Biological Process: activation of protein kinase activity; anoikis; autophagy; axonogenesis; cell cycle arrest; energy reserve metabolic process; establishment of cell polarity; glucose homeostasis; Golgi localization; insulin receptor signaling pathway; negative regulation of cell growth; negative regulation of cell proliferation; positive regulation of autophagy; positive regulation of axonogenesis; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of transforming growth factor beta receptor signaling pathway; positive thymic T cell selection; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of cell growth; regulation of dendrite morphogenesis; regulation of fatty acid biosynthetic process; regulation of protein kinase B signaling cascade; regulation of Wnt receptor signaling pathway; response to DNA damage stimulus; response to ionizing radiation; spermatid development; T cell receptor signaling pathway; tissue homeostasis; vasculature development; Wnt receptor signaling pathway through beta-catenin Disease: Pancreatic Cancer; Peutz-jeghers Syndrome; Testicular Germ Cell Tumor
NCBI Summary:	This gene, which encodes a member of the serine/threonine kinase family, regulates cell polarity and functions as a tumor suppressor. Mutations in this gene have been associated with Peutz-Jeghers syndrome, an autosomal dominant disorder characterized by the growth of polyps in the gastrointestinal tract, pigmented macules on the skin and mouth, and other neoplasms. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008]
UniProt Code:	Q15831
NCBI GenInfo Identifier:	3024670
NCBI Gene ID:	6794
NCBI Accession:	Q15831.1
UniProt Secondary Accession:	Q15831,B2RBX7, E7EW76,
UniProt Related Accession:	Q15831
Molecular Weight:	45,387 Da
NCBI Full Name:	Serine/threonine-protein kinase STK11
NCBI Synonym Full Names:	serine/threonine kinase 11
NCBI Official Symbol:	STK11
NCBI Official Synonym Symbols:	PJS; LKB1; hLKB1
NCBI Protein Information:	serine/threonine-protein kinase STK11
UniProt Protein Name:	Serine/threonine-protein kinase STK11
UniProt Synonym Protein Names:	Liver kinase B1; LKB1; hLKB1; Renal carcinoma antigen NY-REN-19
Protein Family:	Serine/threonine-protein kinase
UniProt Gene Name:	STK11
UniProt Entry Name:	STK11_HUMAN

	Western blot analysis of extracts of various cell lines, using LKB1 antibody (CAB2122) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Western blot analysis of extracts from normal (control) and LKB1 Rabbit pAb knockout (KO) 293T cells, using LKB1 Rabbit pAb antibody (CAB2122) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s.
	Immunohistochemistry of paraffin-embedded human lung cancer using LKB1 antibody (CAB2122) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon carcinoma using LKB1 antibody (CAB2122) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human uterine cancer using LKB1 antibody (CAB2122) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-Phospho-STK11-S334 Antibody (CABP0601)	Human Serine/threonine-protein kinase STK11 (STK11) ELISA Kit
Anti-Phospho-STK11-S428 Antibody (CABP0602)	LKB1 (Phospho-Ser428) Colorimetric Cell-Based ELISA Kit
Anti-Phospho-STK11-T189 Antibody (CABP0603)	LKB1 Colorimetric Cell-Based ELISA Kit
Anti-Phospho-STK11-S428 Antibody (CABP1108)	LKB1 (Phospho-Thr189) Colorimetric Cell-Based ELISA Kit
STK11 Antibody (PACO01024)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)