Anti-PLA2G2D Antibody (CAB7165)

Antibody Name:	Anti-PLA2G2D Antibody
Antibody SKU:	CAB7165
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 21-145 of human PLA2G2D (NP_036532.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100
Reactivity:	Human, Rat
Positive Samples:	Rat lung, Rat spinal cord

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 21-145 of human PLA2G2D (NP_036532.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GILN LNKM VKQV TGKM PILS YWPY GCHC GLGG RGQP KDAT DWCC QTHD CCYD HLKT QGCS IYKD YYRY NFSQ GNIH CSDK GSWC EQQL CACD KEVA FCLK RNLD TYQK RLRF YWRP HCRG QTPG C
Gene ID:	26279
Uniprot:	Q9UNK4
Cellular Location:	Secreted
Calculated MW:	16kDa
Observed MW:	17kDa

Synonyms:

PLA2G2D, PLA2IID, SPLASH, sPLA2-IID, sPLA2S

Background:

This gene encodes a secreted member of the phospholipase A2 family, and is found in a cluster of related family members on chromosome 1. Phospholipase A2 family members hydrolyze the sn-2 fatty acid ester bond of glycerophospholipids to produce lysophospholipids and free fatty acid. This gene may be involved in inflammation and immune response, and in weight loss associated with chronic obstructive pulmonary disease. Alternative splicing results in multiple transcript variants encoding different isoforms.

UniProt Protein Function:	PLA2G2D: PA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. L-alpha-1-palmitoyl-2- linoleoyl phosphatidylethanolamine is more efficiently hydrolyzed than the other phospholipids examined. Belongs to the phospholipase A2 family.
UniProt Protein Details:	Protein type:Lipid Metabolism - alpha-linolenic acid; Lipid Metabolism - linoleic acid; Lipid Metabolism - ether lipid; Phospholipase; Lipid Metabolism - arachidonic acid; Secreted; Secreted, signal peptide; Lipid Metabolism - glycerophospholipid; EC 3.1.1.4 Chromosomal Location of Human Ortholog: 1p36.12 Cellular Component: extracellular region Molecular Function:phospholipase A2 activity; calcium ion binding Biological Process: phospholipid metabolic process; glycerophospholipid biosynthetic process; phosphatidic acid biosynthetic process; inflammatory response; lipid catabolic process
NCBI Summary:	This gene encodes a secreted member of the phospholipase A2 family, and is found in a cluster of related family members on chromosome 1. Phospholipase A2 family members hydrolyze the sn-2 fatty acid ester bond of glycerophospholipids to produce lysophospholipids and free fatty acid. This gene may be involved in inflammation and immune response, and in weight loss associated with chronic obstructive pulmonary disease. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2012]
UniProt Code:	Q9UNK4
NCBI GenInfo Identifier:	20139286
NCBI Gene ID:	26279
NCBI Accession:	Q9UNK4.2
UniProt Secondary Accession:	Q9UNK4,Q9UK01, A8K2Z1, B1AEL9,
UniProt Related Accession:	Q9UNK4
Molecular Weight:	145
NCBI Full Name:	Group IID secretory phospholipase A2
NCBI Synonym Full Names:	phospholipase A2, group IID
NCBI Official Symbol:	PLA2G2D
NCBI Official Synonym Symbols:	SPLASH; sPLA2S; PLA2IID; sPLA2-IID
NCBI Protein Information:	group IID secretory phospholipase A2; GIID sPLA2; secretory phospholipase A2s; phosphatidylcholine 2-acylhydrolase 2D; secretory-type PLA, stroma-associated homolog
UniProt Protein Name:	Group IID secretory phospholipase A2
UniProt Synonym Protein Names:	PLA2IID; Phosphatidylcholine 2-acylhydrolase 2D; Secretory-type PLA, stroma-associated homolog
Protein Family:	Group IID secretory phospholipase
UniProt Gene Name:	PLA2G2D
UniProt Entry Name:	PA2GD_HUMAN

	Western blot analysis of extracts of various cell lines, using PLA2G2D antibody (CAB7165) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of U2OS cells using PLA2G2D antibody (CAB7165). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-PLA2G2D Antibody (CAB6690)	Human PLA2G2D (Phospholipase A2, Group II D) CLIA Kit (HUES01231)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)