The PLA2G2D Antibody (CAB7165) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a secreted member of the phospholipase A2 family, and is found in a cluster of related family members on chromosome 1. Phospholipase A2 family members hydrolyze the sn-2 fatty acid ester bond of glycerophospholipids to produce lysophospholipids and free fatty acid. This gene may be involved in inflammation and immune response, and in weight loss associated with chronic obstructive pulmonary disease. Alternative splicing results in multiple transcript variants encoding different isoforms. RRID AB_2767717 Gene ID 26279 Swiss Prot Synonym SPLASH; sPLA2S; PLA2IID; sPLA2-IID; PLA2G2D
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
PLA2G2D Antibody
SKU:
CAB7165
Size:
100μL, 20μL
Reactivity:
Human, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:2000
IHC-P
1:50 - 1:200
IF
/
ICC
1:10 - 1:100
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SPLASH, sPLA2S, PLA2IID, sPLA2-IID, PLA2G2D
Positive Sample:
Daudi, RPMI 8226, Rat lung, Rat spinal cord
Cellular Localization:
Secreted.
Calculated MW:
17kDa
Observed MW:
22kDa/
This gene encodes a secreted member of the phospholipase A2 family, and is found in a cluster of related family members on chromosome 1. Phospholipase A2 family members hydrolyze the sn-2 fatty acid ester bond of glycerophospholipids to produce lysophospholipids and free fatty acid. This gene may be involved in inflammation and immune response, and in weight loss associated with chronic obstructive pulmonary disease. Alternative splicing results in multiple transcript variants encoding different isoforms. RRID AB_2767717 Gene ID 26279 Swiss Prot Synonym SPLASH; sPLA2S; PLA2IID; sPLA2-IID; PLA2G2D
Purification Method:
Affinity purification
Gene ID:
26279
RRID:
AB_2767717
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PLA2G2D Rabbit pAb (CAB7165) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates / proteins: 25 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of U2OS cells using PLA2G2D Rabbit pAb (CAB7165). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
